The smfBox is an open-source platform for single-molecule FRET

被引：25

作者：

Ambrose, Benjamin ^{[1
]}

Baxter, James M. ^{[1
]}

Cully, John ^{[1
]}

Willmott, Matthew ^{[1
]}

Steele, Elliot M. ^{[2
]}

Bateman, Benji C. ^{[3
]}

Martin-Fernandez, Marisa L. ^{[3
]}

Cadby, Ashley ^{[2
]}

Shewring, Jonathan ^{[1
]}

Aaldering, Marleen ^{[1
]}

Craggs, Timothy D. ^{[1
]}

机构：

[1] Univ Sheffield, Sheffield Inst Nucle Acids, Dept Chem, Sheffield, S Yorkshire, England

[2] Univ Sheffield, Dept Phys, Sheffield, S Yorkshire, England

[3] Rutherford Appleton Lab, Cent Laser Facil, Res Complex Harwell, Oxford, England

来源：

NATURE COMMUNICATIONS | 2020年 / 11卷 / 01期

基金：

英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

ALTERNATING-LASER EXCITATION; DYNAMICS; PRECISION;

D O I：

10.1038/s41467-020-19468-4

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Single-molecule Forster Resonance Energy Transfer (smFRET) is a powerful technique capable of resolving both relative and absolute distances within and between structurally dynamic biomolecules. High instrument costs, and a lack of open-source hardware and acquisition software have limited smFRET's broad application by non-specialists. Here, we present the smfBox, a cost-effective confocal smFRET platform, providing detailed build instructions, open-source acquisition software, and full validation, thereby democratising smFRET for the wider scientific community. Broad uptake of smFRET has been hindered by high instrument costs and a lack of open-source hardware and acquisition software. Here, the authors present the smfBox, a cost-effective open-source platform capable of measuring precise FRET efficiencies between dyes on freely diffusing single molecules.

引用

页数：6

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