Minimal residual disease detected by droplet digital PCR in peripheral blood stem cell grafts has a prognostic impact on high-risk neuroblastoma patients

被引：2

作者：

Nino, Nanako ^{[1
]}

Ishida, Toshiaki ^{[2
]}

Nakatani, Naoko ^{[1
]}

Lin, Kyaw San ^{[1
]}

Win, Kaung Htet Nay ^{[3
]}

Mon, Cho Yee ^{[3
]}

Nishimura, Akihiro ^{[1
]}

Inoue, Shotaro ^{[1
]}

Tamura, Akihiro ^{[1
]}

Yamamoto, Nobuyuki ^{[1
]}

Uemura, Suguru ^{[2
]}

Saito, Atsuro ^{[2
]}

Mori, Takeshi ^{[2
]}

Hasegawa, Daiichiro ^{[2
]}

Kosaka, Yoshiyuki ^{[2
]}

Nozu, Kandai ^{[1
]}

Nishimura, Noriyuki ^{[3
]}

机构：

[1] Kobe Univ, Grad Sch Med, Dept Pediat, Kobe, Hyogo, Japan

[2] Kobe Childrens Hosp, Dept Hematol & Oncol, Kobe, Hyogo, Japan

[3] Kobe Univ, Grad Sch Hlth Sci, Dept Publ Hlth, Kobe, Hyogo, Japan

来源：

HELIYON | 2022年 / 8卷 / 10期

基金：

日本学术振兴会;

关键词：

Neuroblastoma (NB); Minimal residual disease (MRD); Peripheral blood stem cell (PBSC); Neuroblastoma-associated mRNAs (NB-mRNAs); Droplet digital PCR (ddPCR); MARROW DISEASE; CLASSIFICATION; INFORMATION; CHILDREN; SURVIVAL; PUBLICATION; HARVESTS; TIME; RNAS;

D O I：

10.1016/j.heliyon.2022.e10978

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

More than half of high-risk neuroblastoma (NB) patients have experienced relapse due to the activation of chemoresistant minimal residual disease (MRD) even though they are treated by high-dose chemotherapy with autologous peripheral blood stem cell (PBSC) transplantation. Although MRD in high-risk NB patients can be evaluated by quantitative PCR with several sets of neuroblastoma-associated mRNAs (NB-mRNAs), the prognostic significance of MRD in PBSC grafts (PBSC-MRD) is unclear. In the present study, we collected 20 PBSC grafts from 20 high-risk NB patients and evaluated PBSC-MRD detected by droplet digital PCR (ddPCR) with 7NB-mRNAs (CRMP1, DBH, DDC, GAP43, ISL1, PHOX2B, and TH mRNA). PBSC-MRD in 11 relapsed patients was significantly higher than that in 9 non-relapsed patients. Patients with a higher PBSC-MRD had a lower 3-year event-free survival (P = 0.0148). The present study suggests that PBSC-MRD detected by ddPCR with 7NB-mRNAs has a prognostic impact on high-risk NB patients.

引用

页数：6

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