First Report of Pathogenic Bacterium Kalamiella piersonii Isolated from Urine of a Kidney Stone Patient: Draft Genome and Evidence for Role in Struvite Crystallization

被引：17

作者：

Rekha, Punchappady Devasya ^{[1
]}

Hameed, Asif ^{[1
,2
]}

Manzoor, Muhammed A. P. ^{[1
]}

Suryavanshi, Mangesh, V ^{[1
]}

Ghate, Sudeep D. ^{[1
]}

Arun, A. B. ^{[1
]}

Rao, Sneha S. ^{[1
]}

Athmika ^{[1
]}

Bajire, Sukesh Kumar ^{[1
]}

Mujeeburahiman, M. ^{[3
]}

Young, C-C ^{[2
]}

机构：

[1] Yenepoya Deemed Be Univ, Yenepoya Res Ctr, Mangalore 575018, India

[2] Natl Chung Hsing Univ, Coll Agr & Nat Resources, Dept Soil & Environm Sci, Taichung 40227, Taiwan

[3] Yenepoya Deemed Be Univ, Yenepoya Med Coll & Hosp, Dept Urol, Mangalore 575018, India

来源：

PATHOGENS | 2020年 / 9卷 / 09期

关键词：

uropathogen; amino acid metabolism; asparaginase; urea carboxylase; struvite; media alkalization; virulence; synthetic urine; motility and biofilm; lithogenesis; UROPATHOGENIC ESCHERICHIA-COLI; MAXIMUM-LIKELIHOOD; INFECTION; UREASE; PRECIPITATION; SEQUENCES; MOTILITY; INVASION; DATABASE; ASSAY;

D O I：

10.3390/pathogens9090711

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Uropathogenic bacteria are widely distributed in the environment and urinary tract infection is implicated in kidney stone disease. Here, we report on a urease negative bacterium Kalamiella piersonii (strain YU22) isolated from the urine of a struvite stone (MgNH4PO4 center dot 6H(2)O) patient. The closest species, K. piersonii IIIF1SW-P2(T) was reported from International Space Station samples. However, there are no earlier reports on its human association. Using whole genome and experimental analysis, its involvement in urinary tract colonization and struvite crystallization was explored. The strain YU22 showed many virulence factors that are needed for host cell invasion and colonization including cell adhesion factors, swimming and swarming motilities, biofilm and siderophore among others. In vitro infection studies in HEK-293T cells demonstrated the host cell attachment and killing. It was able to utilize amino acids as sole carbon source and showed growth in synthetic and healthy urine establishing metabolic adaptation to urinary tract. Increased pH and availability of ammonium ions from amino acid breakdown promoted struvite crystallization. The results from this study support the involvement of urease negative uropathogen in the struvite lithogenesis. Further studies on other isolates of K. peirsonii are warranted to assess its health risks.

引用

页码：1 / 20

页数：20