Development and use of simple sequence repeat SSR markers in Rubus species

被引：49
作者：
Graham, J [1 ]
Smith, K [1 ]
Woodhead, M [1 ]
Russell, J [1 ]
机构：
[1] Scottish Crop Res Inst, Dundee DD2 5DA, Scotland
来源：
MOLECULAR ECOLOGY NOTES | 2002年 / 2卷 / 03期
关键词：
microsatellite primers; Rubus; red raspberry;
D O I：
10.1046/j.1471-8286.2002.00203.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The isolation of polymorphic codominant microsatellite markers in Rubus and in particular red raspberry will provide a tool to investigate gene flow between cultivated and wild raspberries. Microsatellite loci were isolated by screening a PstI size selected genomic library with AC((13)) and AG((13)). Positive clones were sequenced and primer pairs designed to the sequences flanking identified SSRs. One primer of each pair was fluorescently labelled to facilitate polymerase chain reaction (PCR) product identification on an automated DNA sequencer. We describe 10 polymorphic microsatellite loci developed and demonstrate their usefulness in different Rubus species.
引用
收藏
页码：250 / 252
页数：3
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