Comparison of a commercial real-time PCR assay, RealCycler® PJIR kit, progenie molecular, to an in-house real-time PCR assay for the diagnosis of Pneumocystis jirovecii infections

被引：14

作者：

Guillaud-Saumur, Thibaud ^{[1
,2
]}

Nevez, Gilles ^{[1
,2
]}

Bazire, Amelie ^{[3
]}

Virmaux, Michele ^{[2
]}

Papon, Nicolas ^{[4
]}

Le Gal, Solene ^{[1
,2
]}

机构：

[1] Brest Univ Hosp, Lab Parasitol & Mycol, Brest, France

[2] Univ Brest, GEIHP EA 3142, Brest, France

[3] Brest Univ Hosp, Med Intens Care Unit, Brest, France

[4] Univ Angers, GEIHP EA 3142, Angers, France

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2017年 / 87卷 / 04期

关键词：

Pneumocystis jirovecii; Real-time PCR; Commercial kit; Comparison of methods; COLONIZATION; CARINII;

D O I：

10.1016/j.diagmicrobio.2017.01.011

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

We compared the RealCycler (R) PJIR kit (Progenie Molecular), available in Europe, to an in-house real-time PCR assay for the diagnosis of Pneumocystis jirovecii infections. Excellent agreement was found (concordance rate, 97.4%; Cohen's kappa, 0.918 > 0.8) showing that this commercial assay represents an alternative method for the diagnosis of P. jirovecii infections. (C) 2017 Elsevier Inc. All rights reserved.

引用

页码：335 / 337

页数：3

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