Monoclonal antibodies to gonadotropin-releasing hormone (GnRH) inhibit binding of the hormone to its receptor

被引：15

作者：

Gangatirkar, P

Gangadharan, S

Narendranath, A

Nagpal, S

Salunke, DM

Karande, AA ^{[1
]}

机构：

[1] Indian Inst Sci, Dept Biochem, Bangalore 560012, Karnataka, India

[2] Natl Inst Immunol, New Delhi 110067, India

来源：

HYBRIDOMA AND HYBRIDOMICS | 2002年 / 21卷 / 04期

关键词：

D O I：

10.1089/153685902760213895

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Monoclonal antibodies (MAbs) specific to gonadotropin-releasing hormone (GnRH) were obtained using different strategies of conjugation of the peptide to carrier protein and immunization. Of several antibodies obtained, two, namely F1D3C5 and E2D2 bound GnRH in solution phase. Though the epitopes corresponding to the two overlapped, there was a one amino acid shift in the core epitope. These two antibodies were characterized with respect to inhibition of GnRH induced responses in rat pituitary cultures and alpha-T3.1 mouse gonadotrope cell line.

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收藏

页码：281 / 286

页数：6

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