Towards efficient biocatalysts: photo-immobilization of a lipase on novel lysozyme amyloid-like nanofibrils

被引：24

作者：

Chaves, Silvina ^{[1
,2
]}

Pera, Licia M. ^{[3
,4
]}

Luis Avila, Cesar ^{[1
,2
]}

Romero, Cintia M. ^{[3
,4
]}

Baigori, Mario ^{[3
,4
]}

Moran Vieyra, F. Eduardo ^{[5
]}

Borsarelli, Claudio D. ^{[5
]}

Chehin, Rosana N. ^{[1
,2
]}

机构：

[1] UNT, Inst Super Invest Biol INSIBIO, CONICET UNT, Chacabuco 461,T4000ILI, San Miguel De Tucuman, Tucuman, Argentina

[2] UNT, Inst Quim Biol Dr Bernabe Bloj, Fac Bioquim Quim & Farm, Chacabuco 461,T4000ILI, San Miguel De Tucuman, Tucuman, Argentina

[3] Planta Piloto Procesos Ind Microbiol PROIMI CONIC, Av Belgrano & Pasaje Caseros,T4001 MVB, San Miguel De Tucuman, Tucuman, Argentina

[4] Univ Nacl Tucuman, Fac Bioquim, Quim, Farm, Ayacucho 471,T4000INI, RA-4000 San Miguel De Tucuman, Tucuman, Argentina

[5] UNSE, INBIONATEC CONICET, Inst Bionanotecnol, RN 9,Km 1125,G4206XCP, Santiago Del Estero, Argentina

来源：

RSC ADVANCES | 2016年 / 6卷 / 11期

关键词：

EGG-WHITE LYSOZYME; FIBRIL FORMATION; ENZYME IMMOBILIZATION; MOLECULAR-DYNAMICS; INFRARED-SPECTROSCOPY; ASSEMBLY PATHWAYS; AQUEOUS CONTENT; LYTIC ACTIVITY; CROSS-LINKING; IN-VITRO;

D O I：

10.1039/c5ra19590j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Herein, we report the preparation and characterization of a new biocatalyst based on the photo-immobilization of a lipase onto hybrid protein : sugar nanofibrils. The nanofibrils are obtained through the aggregation of hen white egg lysozyme induced by the highly sulfated glycosaminoglycan heparin. The new hybrid nanomaterial could be easily functionalized using photochemical reaction to attach lipases through dityrosine covalent bonds. Compared to the free enzyme, the photo-immobilized lipase has better thermostability and enhanced resistance to non-conventional environment. Structural and morphological characterization of the nanofibrils shows that they are compatible with amyloid-like aggregates. In addition, the supramolecular arrangement of heparin and lysozyme within the building unit of the nanofibrils is also proposed. The procedure reported herein could be useful to design a new generation of insoluble biocatalyst by a single photo-click step which is definitely cleaner and faster than conventional chemical cross-linked procedures.

引用

页码：8528 / 8538

页数：11

共 77 条

[1] STRUCTURE AND THERMAL-DENATURATION OF CRYSTALLINE AND NONCRYSTALLINE CYTOCHROME-OXIDASE AS STUDIED BY INFRARED-SPECTROSCOPY [J].