A stop-EGFP transgenic mouse to detect clonal cell lineages generated by mutation

被引：35

作者：

Ro, S ^{[1
]}

Rannala, B ^{[1
]}

机构：

[1] Univ Alberta, Dept Med Genet, Edmonton, AB T6G 2H7, Canada

来源：

EMBO REPORTS | 2004年 / 5卷 / 09期

关键词：

clonal cell lineage; enhanced green fluorescent protein; epidermal stem cell; in vivo imaging; mutation;

D O I：

10.1038/sj.embor.7400218

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The investigation of cell lineages and clonal organization in tissues is facilitated by techniques that allow labelling of clonal cell lineages. Here, we describe a novel transgenic mouse that allows clonal cell lineages to be traced in virtually any tissue. A green fluorescent cell lineage is generated by a random mutation at an enhanced green fluorescent protein gene that carries a premature stop codon, ensuring clonality. The transgenic system allows efficient detection of mutations and stem-cell fate mapping in the epidermis using live mice, as well as in the kidney and liver post-mortem. Cell lineages that descended from single epidermal stem cells were found to be capable of generating three adjacent corneocytes using the system, providing evidence for horizontal migration of epidermal cells between epidermal proliferative units (EPUs), in contrast to the classical EPU model. The transgenic mouse system is expected to provide a novel tool for stem-cell lineage studies.

引用

页码：914 / 920

页数：7

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