Genetic diversity studies of Papaya meleira virus

被引：6

作者：

Daltro, Cleidiane B. ^{[1
]}

Medeiros Abreu, Emanuel Felipe ^{[2
]}

Lima Aragao, Francisco Jose ^{[3
]}

Andrade, Eduardo C. ^{[2
]}

机构：

[1] Univ Fed Reconcavo Bahia, Ctr Ciencias Agr Ambientais & Biol, BR-44380000 Cruz Das Almas, BA, Brazil

[2] Embrapa Mandioca & Fruticultura, BR-44380000 Cruz Das Almas, BA, Brazil

[3] Embrapa Recursos Genet & Biotecnol, BR-70770917 Brasilia, DF, Brazil

来源：

TROPICAL PLANT PATHOLOGY | 2014年 / 39卷 / 01期

关键词：

Carica papaya; Totiviridae; sticky disease; variability; viruses;

D O I：

10.1590/S1982-56762014000100013

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Papaya (Carica papaya) is a fruit crop of great economic and social importance for Brazil and other papaya-producing countries. Brazil is the second largest producer in the world. The papaya sticky disease, caused by Papaya meleira virus (PMeV), has caused great losses in the major Brazilian papaya-producing states. In order to estimate the genetic diversity of PMeV, latex samples were collected from papaya plants in the states of Bahia, Esprito Santo, Pernambuco, Ceara and Rio Grande do Norte, and total RNA was extracted. Specific primer for the replicase region allowed the amplification, by RT-PCR, of a fragment of approximately 560 bp from 31 isolates. The sequence analysis indicated a level of conservation greater than 88% among isolates. Furthermore, comparative analyzes indicated that PMeV has similarity with mycoviruses of the family Totiviridae. This phylogenetic relationship was reinforced by the presence of conserved motifs within in the RdRp regions from mycoviruses.

引用

页码：104 / 108

页数：5

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