Utilization of enzyme extract self-encapsulated within polypyrrole in sensitive detection of catechol

被引:16
作者
Apetrei, Roxana-Mihaela [1 ]
Carac, Geta [2 ]
Bahrim, Gabriela [1 ]
Camurlu, Pinar [3 ]
机构
[1] Dunarea de Jos Univ Galati, Fac Food Sci & Engn, Domneasca St 47, RO-800008 Galati, Romania
[2] Dunarea de Jos Univ Galati, Fac Sci & Environm, Domneasca St 47, RO-800008 Galati, Romania
[3] Akdeniz Univ, Dept Chem, TR-07058 Antalya, Turkey
关键词
Amperometric biosensors; Sensitive catechol detection; Crude enzyme extract; Trametes pubescens; Ppy biosynthesis; FLOW-INJECTION DETERMINATION; PHENOLIC-COMPOUNDS; AMPEROMETRIC DETERMINATION; IMMOBILIZED LACCASE; POLYPHENOL OXIDASE; CONDUCTING POLYMER; GLUCOSE-OXIDASE; BIOSENSOR; TYROSINASE; PEROXIDASE;
D O I
10.1016/j.enzmictec.2019.04.015
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Utilization of polyphenol oxidases (laccase, tyrosinase) in biosensor technology is an efficient approach towards phenol detection, which is significant in numerous fields such as environmental monitoring, food industry etc. The use of crude extract instead of pure enzyme eliminates the need for costly and laborious processes of enzyme separation and purification. This study employs polyphenol oxidase extract, biosynthesized by white-rot fungi Trametes pubescens (TP) for the development of amperometric biosensors for catechol detection. The catalytic activity of the crude extract was firstly used to induce the bio-synthesis of conducting polymer - polypyrrole (Ppy), resulting in the self-encapsulation of the enzyme extract within the conducting material. The viability and biological integrity of the enzyme extract was preserved after the synthesis and was able to efficiently detect phenolic compounds such as catechol. Comparative evaluations between the biosynthesized Ppy based biosensor (bio-Ppy) and the biosensor based on bio-PPy with additional enzyme extract (bio-Ppy-TP) were performed. Lastly, the performance of these two biosensors was compared with that of a third one, based on chemically synthesized Ppy with enzyme extract (them-Ppy-7P). All three types of biosensors proved high efficiency for catechol detection at low concentration (1-60 mu M) and were employed for real sample detection in fruit wines showing linear correlation with the spectrophotometric results obtained with the Folin-Ciocalteau standard test.
引用
收藏
页码:34 / 39
页数:6
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