Spectroscopic study of gamma irradiation effect on the molecular structure of bovine serum albumin

被引:14
作者
Zarei, Hajar [1 ]
Bahreinipour, Mostean [1 ]
Eskandari, Khadijeh [2 ]
MousaviZarandi, Seyed-Ali [1 ]
Ardestani, Susan Kaboudanian [3 ]
机构
[1] Amirkabir Univ Technol, Dept Energy Engn & Phys, Fac Phys, Tehran, Iran
[2] Baqiyatallah Univ Med Sci, Nanobiotechnol Res Ctr, Tehran, Iran
[3] Univ Tehran, Inst Biochem & Biophys, Immunol Lab, Tehran 14174, Iran
关键词
Irradiation; Bovine serum albumin; Spectroscopy; Electrophoresis; Dynamic light scattering; Electrophoretic light scattering; CIRCULAR-DICHROISM SPECTROSCOPY; SECONDARY-STRUCTURE ANALYSIS; PROTEIN DAMAGE; RADIATION; DENATURATION; MECHANISMS; RADIOLYSIS; DOMAIN;
D O I
10.1016/j.vacuum.2016.11.029
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
The effect of gamma radiation on the molecular structure, size distribution and surface charge of bovine serum albumin (BSA) was studied by spectroscopic techniques. The first structure of non-irradiated and irradiated BSA were investigated by UV-Vis spectroscopy and electrophoresis (SDS-PAGE). Additionally, the secondary and tertiary structural changes of BSA were studied by circular dichroism (CD) and fluorescence spectroscopy, respectively. Dynamic light scattering (DLS) and electrophoretic light scattering (ELS) was used for clarify of size and surface charge of BSA. The results indicate that the first structure of BSA is preserved, while the secondary and tertiary structures have changed significantly. The result of CD studies shows an 8% decrease in a-helix and an increase in other secondary structures for irradiated BSA in comparison to non-irradiated ones. Moreover, DLS and ELS displayed the decrease in the size and surface charge of irradiated BSA. The aggregation of irradiated BSA was also confirmed by fluorescence spectroscopy. The ELS results as an additional data confirm the aggregation of protein. The results demonstrate that doses close to therapeutic ones can lead to structural changes in macromolecules as well as the aggregation of polypeptide chain but without the fragmentation. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码:91 / 96
页数:6
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