Site-directed mutagenesis by the megaprimer PCR method: Variations on a theme for simultaneous introduction of multiple mutations

被引：23

作者：

Angelaccio, S ^{[1
]}

di Patti, MCB ^{[1
]}

机构：

[1] Univ Roma La Sapienza, Dipartimento Sci Biochim A Rossi Fanelli, I-00185 Rome, Italy

来源：

ANALYTICAL BIOCHEMISTRY | 2002年 / 306卷 / 02期

关键词：

D O I：

10.1006/abio.2002.5689

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

[No abstract available]

引用

页码：346 / 349

页数：4

共 10 条

[1]

BARIK S, 1991, BIOTECHNIQUES, V10, P489

[2] Site-directed mutagenesis by combined chain reaction [J].

Bi, WL ;

Stambrook, PJ .

ANALYTICAL BIOCHEMISTRY, 1998, 256 (01) :137-140

[3] EFFICIENT AMPLIFICATION USING MEGAPRIMER BY ASYMMETRIC POLYMERASE CHAIN-REACTION [J].

DATTA, AK .

NUCLEIC ACIDS RESEARCH, 1995, 23 (21) :4530-4531

[4] The essential role of Glu-185 and Tyr-354 residues in the ferroxidase activity of Saccharomyces cerevisiae Fet3 [J].

di Patti, MCB ;

Felice, MR ;

Camuti, AP ;

Lania, A ;

Musci, G .

FEBS LETTERS, 2000, 472 (2-3) :283-286

[5] Simultaneous introduction of multiple mutations using overlap extension PCR [J].

Ge, LM ;

Rudolph, P .

BIOTECHNIQUES, 1997, 22 (01) :28-&

[6] Site-directed mutagenesis techniques in the study of Escherichia coli serine hydroxymethyltransferase [J].

Iurescia, S ;

Condo, I ;

Angelaccio, S ;

DelleFratte, S ;

Bossa, F .

PROTEIN EXPRESSION AND PURIFICATION, 1996, 7 (03) :323-328

[7] A ONE-STEP POLYMERASE CHAIN-REACTION SITE-DIRECTED MUTAGENESIS METHOD FOR LARGE GENE-CASSETTES WITH HIGH-EFFICIENCY, YIELD, AND FIDELITY [J].

LING, MF ;

ROBINSON, BH .

ANALYTICAL BIOCHEMISTRY, 1995, 230 (01) :167-172

[8] Approaches to DNA mutagenesis: An overview [J].

Ling, MM ;

Robinson, BH .

ANALYTICAL BIOCHEMISTRY, 1997, 254 (02) :157-178

[9]

ROUWENDAL GJA, 1993, BIOTECHNIQUES, V15, P68

[10]

SARKAR G, 1990, BIOTECHNIQUES, V8, P404

← 1 →