Galleria mellonella larvae allow the discrimination of toxic and non-toxic chemicals

被引：110

作者：

Allegra, Enrico ^{[1
]}

Titball, Richard W. ^{[2
]}

Carter, John ^{[1
]}

Champion, Olivia L. ^{[3
]}

机构：

[1] Envigo Ltd, Huntingdon, Cambs, England

[2] Univ Exeter, Biosci, Exeter EX4 4QP, Devon, England

[3] Biosyst Technol Ltd Colleton Crescent, Exeter EX2 4DG, Devon, England

来源：

CHEMOSPHERE | 2018年 / 198卷

基金：

英国国家替代、减少和改良动物研究中心;

关键词：

Galleria mellonella; Toxicity testing; Cell culture; Globally Harmonised system; IN-VIVO MODEL;

D O I：

10.1016/j.chemosphere.2018.01.175

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The acute toxicities of 19 chemicals were assessed using G. mellonella larvae. The results obtained were compared against LD50 values derived from in vitro cytotoxicity tests and against in vivo acute oral LD50 values. In general, cell culture systems overestimated the toxicity of chemicals, especially low toxicity chemicals. In contrast, toxicity testing in G. mellonella larvae was found to be a reliable predictor for low toxicity chemicals. For the 9 chemicals tested which were assigned to Globally Harmonised System (GHS) category 5, the toxicity measured in G. mellonella larvae was consistent with their GHS categorisation but cytotoxicity measured in 3T3 or NHK cells predicted 4 out of 9 chemicals as having low toxicity. A more robust assessment of the likely toxicity of chemicals in mammals could be made by taking into account their toxicities in both cell cultures and in G. mellonella larvae. (C) 2018 Elsevier Ltd. All rights reserved.

引用

页码：469 / 472

页数：4

共 17 条

[1] EFFECT OF OXYGEN ON CYANIDE POISONING IN INSECTS [J].