Nanostructured FeS as a Mimic Peroxidase for Biocatalysis and Biosensing

被引:294
作者
Dai, Zhihui [1 ]
Liu, Shaohua [1 ]
Bao, Jianchun [1 ]
Jui, Huangxian [2 ]
机构
[1] Nanjing Normal Univ, Coll Chem & Environm Sci, Jiangsu Key Lab Biofunct Mat, Nanjing, Peoples R China
[2] Nanjing Univ, Dept Chem, MOE Key Lab Analyt Chem Life Sci, Nanjing 210093, Peoples R China
基金
中国国家自然科学基金;
关键词
amperometric biosensors; biosensors; enzyme catalysis; FeS nanostructure; peroxide mimic; DIRECT ELECTRON-TRANSFER; PRUSSIAN-BLUE; HYDROGEN-PEROXIDE; HORSERADISH-PEROXIDASE; CARBON NANOTUBES; NANOPARTICLES; MATRIX; H2O2; ELECTROCHEMISTRY; IMMOBILIZATION;
D O I
10.1002/chem.200802158
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Artificial enzyme mimics have attracted considerable interest due to easy denaturation and leakage of enzymes during their storage and immobilization procedure. Herein we describe the design of a novel mimic peroxidase, a nanostructure of sheet-like FeS prepared by a simple micelle-assisted synthetic method. Such a nanostructure has a large specific surface area and high peroxidase-like activity, and was thus further used as a mimic enzyme for the development of biocatalysts and amperometric biosensors. The sheet-like FeS nanostructure showed typical Michaelis-Menten kinetics and good affinity to both H2O2 and 3,3',5,5'-tetramethyl benzidine. At pH 7.0 the constructed amperometric sensor showed a linear range for the detection of H2O2 from 0.5 to 150 mu M with a correlation coefficient of 0.9998 without any electron transfer mediator. ne H2O2 sensor based on the sheet-like FeS showed more sensitive response than those based on spherical FeS nanostructure, and resulted in a better stability than horseradish peroxidase when they were exposed to solutions with different pH values and temperatures. These excellent properties made the sheet-like nanostructured FeS powerful tools for a wide range of potential applications as ail "artificial peroxidase" as biosensors and biotechnology.
引用
收藏
页码:4321 / 4326
页数:6
相关论文
共 53 条
[1]   Electronic structure of the antiferromagnetic B1-structured FeO -: art. no. 165111 [J].
Alfredsson, M ;
Price, GD ;
Catlow, CRA ;
Parker, SC ;
Orlando, R ;
Brodholt, JP .
PHYSICAL REVIEW B, 2004, 70 (16) :1-6
[2]  
[Anonymous], 2002, Angew. Chem. Int. Ed, DOI DOI 10.1038/NMAT1251
[3]   Role of different counterions and size of micelle in concentration dependence micellar structure of ionic surfactants [J].
Aswal, VK ;
Goyal, PS .
CHEMICAL PHYSICS LETTERS, 2003, 368 (1-2) :59-65
[4]   Rationally designed nanostructures for surface-enhanced Raman spectroscopy [J].
Banholzer, Matthew J. ;
Millstone, Jill E. ;
Qin, Lidong ;
Mirkin, Chad A. .
CHEMICAL SOCIETY REVIEWS, 2008, 37 (05) :885-897
[5]   Designing stable redox-active surfaces:: Chemical attachment of an osmium complex to glassy carbon electrodes prefunctionalized by electrochemical reduction of an in situ-generated aryldiazonium cation [J].
Boland, Susan ;
Barriere, Frederic ;
Leech, Donal .
LANGMUIR, 2008, 24 (12) :6351-6358
[6]   POLYOL RECOGNITION BY A STEROID-CAPPED PORPHYRIN - ENHANCEMENT AND MODULATION OF MISFIT GUEST BINDING BY ADDED WATER OR METHANOL [J].
BONARLAW, RP ;
SANDERS, JKM .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1995, 117 (01) :259-271
[7]   BIOMIMETIC CHEMISTRY AND ARTIFICIAL ENZYMES - CATALYSIS BY DESIGN [J].
BRESLOW, R .
ACCOUNTS OF CHEMICAL RESEARCH, 1995, 28 (03) :146-153
[8]   Direct electron transfer of cytochrome c immobilized on a NaY zeolite matrix and its application in biosensing [J].
Dai, ZH ;
Liu, SQ ;
Ju, HX .
ELECTROCHIMICA ACTA, 2004, 49 (13) :2139-2144
[9]   Electrocatalytic H2O2 amperometric detection using gold nanotube electrode ensembles [J].
Delvaux, M ;
Walcarius, A ;
Demoustier-Champagne, S .
ANALYTICA CHIMICA ACTA, 2004, 525 (02) :221-230
[10]   Surfactant promoting effects on clathrate hydrate formation: Are micelles really involved? [J].
Di Profio, P ;
Arca, S ;
Germani, R ;
Savelli, G .
CHEMICAL ENGINEERING SCIENCE, 2005, 60 (15) :4141-4145