Comparison of different antibodies for detection of progesterone receptor in breast cancer

被引:73
作者
Press, M
Spaulding, B
Groshen, S
Kaminsky, D
Hagerty, M
Sherman, L
Christensen, K
Edwards, DP
机构
[1] Univ Colorado, Hlth Sci Ctr, Dept Pathol, Denver, CO 80262 USA
[2] Eisenhower Med Ctr, Dept Pathol, Palm Desert, CA USA
[3] DAKO Corp, Carpinteria, CA 93013 USA
[4] Univ So Calif, Norris Comprehens Canc Ctr, Los Angeles, CA 90033 USA
[5] Univ So Calif, Sch Med, Dept Pathol, Los Angeles, CA 90033 USA
关键词
progesterone receptor; breast cancer; monoclonal antibody; immunohistochemistry; aparaffin tissue sections;
D O I
10.1016/S0039-128X(02)00039-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Monoclonal antibodies directed against human estrogen receptor (ER) and progesterone receptor (PR) have been used extensively for biochemical and immunohistochemical detection of receptors independent of hormone-binding assays. These antibodies have been valuable both for experimental work and for detection of receptors in clinical breast cancer specimens. The purpose of this study was to characterize the sensitivity and specificity of different antibodies for detection of PR by immunohistochemistry (IHC) of formalin-fixed paraffin breast carcinoma sections. The panel of twelve antibodies included two new ones (PgR636 and PgR1294) produced prospectively to be resistant to formalin fixation and paraffin embedding. Fifty-nine breast carcinomas, having known PR levels by biochemical ligand-binding assay, were used to prepare multitumor paraffin-embedded tissue blocks for characterization of the PR antibodies. Of all the antibodies tested, both PgR636 and PgR1294 stained the highest percentage of breast carcinomas known to be positive by the biochemical assay (95-98%) and they exhibited the highest concordance with the biochemical assay (88-90%). The PgR636 and PgR1294 antibodies, along with one other, PR 88, also gave the highest intensity of nuclear staining, while PgR636 and PgR1294 stained the highest mean percentage of tumor cell nuclei. Antigen retrieval was not necessary for PR immunostaining by PgR636 and PgR1294 in most tumors and other tissues examined, but did slightly increase the staining intensity. The majority of the other antibodies tested were highly dependent on antigen retrieval; only PR 88 and KD 68 antibodies approached the performance of PgR636 and PgR1294 without antigen retrieval. These results indicate that PgR636 and PgR1294 are optimal antibodies for IHC detection of PR in routine paraffin tissue blocks. (C) 2002 Elsevier Science Inc. All rights reserved.
引用
收藏
页码:799 / 813
页数:15
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