Three-factor response surface optimization of the production of intracellular dextran dextrinase by Gluconobacter oxydans

被引：13
作者：
Naessens, A
Vercauteren, R
Vandamme, EJ
机构：
[1] State Univ Ghent, Dept Biochem & Mocrobial Technol, B-9000 Ghent, Belgium
[2] Cerestar Gargill TDC Food Europe, B-1800 Vilvoorde, Belgium
来源：
PROCESS BIOCHEMISTRY | 2004年 / 39卷 / 10期
关键词：
dextran dextrinase; Gluconobacter oxydans; central composite design; medium optimization;
D O I：
10.1016/j.procbio.2003.08.001
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The production of intracellular dextran dextrinase (DDase) by Gluconobacter oxydans ATCC 11894 was studied in fermentations with various carbon and nitrogen sources. Glycerol and mycological peptone resulted in highest enzyme yields. The effect of glycerol concentration, mycological peptone concentration and initial pH on DDase production was investigated by means of a five-level three-factor central composite rotatable design (CCRD). Only the peptone concentration showed a significant linear main effect on enzyme production. Optimum fermentation parameters were 20.59 g/l of glycerol, 6.67 g/l of mycological peptone and an initial pH of 6.14. The predicted DDase yield was 0.207 U/ml, whereas an actual experimental value of 0.208 +/- 0.025 U/ml was obtained. (C) 2003 Elsevier Ltd. All rights reserved.
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页码：1299 / 1304
页数：6
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