Growth enhancement and food conversion efficiency of transgenic fish Labeo rohita

被引：35

作者：

Venugopal, T ^{[1
]}

Anathy, V ^{[1
]}

Kirankumar, S ^{[1
]}

Pandian, TJ ^{[1
]}

机构：

[1] Madurai Kamaraj Univ, Dept Genet, Ctr Adv Studies Funct Genom, Sch Biol Sci, Madurai 625021, Tamil Nadu, India

来源：

JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL AND INTEGRATIVE PHYSIOLOGY | 2004年 / 301A卷 / 06期

关键词：

D O I：

10.1002/jez.a.78

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Three family lines of fast growing transgenic rohu Labeo rohita (rohu) were generated by electroporated-sperm-mediated transfer of the vectors harboring CMV promoter or grass carp beta-actin promoter fused to endogenous rohu GH (rGH) CDNA. The gene transfer efficiency was 25%. The transgenic rohu (family line 1) with CMV promoter showed a growth enhancement of four times normal size, whereas those (family lines 2 and 3) generated with beta-actin promoter grew 4.5 and 5.8 times faster than their respective control siblings. Southern analysis confirmed the transgene extrachromosomal (Te) persistence until the 60th week in family 1. The individuals of family lines 2 and 3, however, showed integration (Ti), as well as persistence as extarchromosomal copies (Te) until the age of 30 weeks. Mosaicism of the transgene was shown at the levels of its presence and expression. The ectopic expression of rGH mRNA was confirmed by RT-PCR. Feeding experiments revealed that the transgenic rohu ate food at a lower rate but grew more efficiently than their control siblings. (C) 2004 Wiley-Liss, Inc.

引用

页码：477 / 490

页数：14

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