Origin and diversity of alginate lyases of families PL-5 and-7 in Sphingomonas sp strain A1

被引:54
作者
Miyake, O [1 ]
Ochiai, A [1 ]
Hashimoto, W [1 ]
Murata, K [1 ]
机构
[1] Kyoto Univ, Grad Sch Agr, Dept Basic & Appl Mol Biotechnol, Uji, Kyoto 6110011, Japan
关键词
D O I
10.1128/JB.186.9.2891-2896.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Sphingonionas sp. strain A1 has three endotype alginate lyases (A1-1, A1-II [family PL-7], and A1-III [family PL-5]), each of which is encoded by a single gene. In addition to those of these lyases, a gene (the A1-II' gene) showing significant identity with the A1-II gene was present in the bacterial genome and coded for an alginate lyase with broad substrate specificity. Since no expression of A1-II' was observed even in bacterial cells grown on alginate, the A1-II' gene was thought to be a silent gene derived from the A1-II gene, presumably through duplication, modification, and translocation.
引用
收藏
页码:2891 / 2896
页数:6
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