Inducing precocious anaphase in cultured mammalian cells

被引：34
作者：
Canman, JC
Salmon, ED
Fang, GW
机构：
[1] Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA
[2] Stanford Univ, Dept Biol Sci, Stanford, CA 94305 USA
来源：
CELL MOTILITY AND THE CYTOSKELETON | 2002年 / 52卷 / 02期
关键词：
D O I：
10.1002/cm.10032
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The spindle checkpoint, which prevents anaphase onset upon spindle damage or incorrect chromosome alignment, presents a problem for experimental analysis of protein function in anaphase and cytokinesis. This is because the functional disruption of many proteins before anaphase onset can activate this checkpoint, preventing anaphase and subsequent cell cycle events. This paper compares new and old methods of overriding the spindle checkpoint in prometaphase mammalian tissue culture cells. (C) 2002 Wiley-Liss. Inc.
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页码：61 / 65
页数：5
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