FlgM is a primary regulator of sigma(D) activity, and its absence restores motility to a sinR mutant

被引：46

作者：

Fredrick, K ^{[1
]}

Helmann, JD ^{[1
]}

机构：

[1] CORNELL UNIV,MICROBIOL SECT,ITHACA,NY 14853

来源：

JOURNAL OF BACTERIOLOGY | 1996年 / 178卷 / 23期

关键词：

D O I：

10.1128/jb.178.23.7010-7013.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have used mini-Tn10 mutagenesis to identify negative regulators of sigma(D) activity, Nine independent insertions were mapped to five genes: flgM, flgK, fliD, fliS, and fliT, suggesting that FlgM export is regulated similarly in Bacillus subtilis and Salmonella typhimurium. We show that a deletion of flgM can restore sigma(D) activity to a sinR null mutant of B, subtilis, although fla/che operon expression is affected by neither SinR nor FlgM.

引用

收藏

页码：7010 / 7013

页数：4

相关论文

共 31 条

[1] COUPLING OF FLAGELLIN GENE-TRANSCRIPTION TO FLAGELLAR ASSEMBLY IN BACILLUS-SUBTILIS [J].

BARILLA, D ;

CARAMORI, T ;

GALIZZI, A .

JOURNAL OF BACTERIOLOGY, 1994, 176 (15) :4558-4564

[2] Role of FlgM in sigma(D)-dependent gene expression in Bacillus subtilis [J].

Caramori, T ;

Barilla, D ;

Nessi, C ;

Sacchi, L ;

Galizzi, A .

JOURNAL OF BACTERIOLOGY, 1996, 178 (11) :3113-3118

[3] THE BACILLUS-SUBTILIS SIGMA(D)-DEPENDENT OPERON ENCODING THE FLAGELLAR PROTEINS FLID, FLIS, AND FLIT [J].

CHEN, L ;

HELMANN, JD .

JOURNAL OF BACTERIOLOGY, 1994, 176 (11) :3093-3101

[4] PROMOTER ARCHITECTURE IN THE FLAGELLAR REGULON OF BACILLUS-SUBTILIS - HIGH-LEVEL EXPRESSION OF FLAGELLIN BY THE SIGMA(D) RNA-POLYMERASE REQUIRES AN UPSTREAM PROMOTER ELEMENT [J].

FREDRICK, K ;

CARAMORI, T ;

CHEN, YF ;

GALIZZI, A ;

HELMANN, JD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (07) :2582-2586

[5] NEGATIVE REGULATORY LOCI COUPLING FLAGELLIN SYNTHESIS TO FLAGELLAR ASSEMBLY IN SALMONELLA-TYPHIMURIUM [J].

GILLEN, KL ;

HUGHES, KT .

JOURNAL OF BACTERIOLOGY, 1991, 173 (07) :2301-2310

[6] A SYMMETRICAL 6-BASE-PAIR TARGET SITE SEQUENCE DETERMINES TN10 INSERTION SPECIFICITY [J].

HALLING, SM ;

KLECKNER, N .

CELL, 1982, 28 (01) :155-163

[7] ALTERNATIVE SIGMA FACTORS AND THE REGULATION OF FLAGELLAR GENE-EXPRESSION [J].

HELMANN, JD .

MOLECULAR MICROBIOLOGY, 1991, 5 (12) :2875-2882

[8] SENSING STRUCTURAL INTERMEDIATES IN BACTERIAL FLAGELLAR ASSEMBLY BY EXPORT OF A NEGATIVE REGULATOR [J].

HUGHES, KT ;

GILLEN, KL ;

SEMON, MJ ;

KARLINSEY, JE .

SCIENCE, 1993, 262 (5137) :1277-1280

[9]

Iyoda S, 1995, MOL GEN GENET, V249, P417

[10] HIGH-LEVEL TRANSCRIPTION OF THE MAJOR BACILLUS-SUBTILIS AUTOLYSIN OPERON DEPENDS ON EXPRESSION OF THE SIGMA D-GENE AND IS AFFECTED BY A SIN (FLAD) MUTATION [J].

KURODA, A ;

SEKIGUCHI, J .

JOURNAL OF BACTERIOLOGY, 1993, 175 (03) :795-801

← 1 2 3 4 →