A microbial biosensor based on Lactobacillus delbruecki sp bacterial cells for simultaneous determination of lactic and pyruvic acid

被引：17

作者：

Canbay, Erhan ^{[1
]}

Habip, Alper ^{[1
]}

Kara, Goknur ^{[1
]}

Eren, Zeynep ^{[1
]}

Akyilmaz, Erol ^{[1
]}

机构：

[1] Ege Univ, Fac Sci, Dept Biochem, TR-35100 Bornova, Turkey

来源：

FOOD CHEMISTRY | 2015年 / 169卷

关键词：

Microbial biosensor; Lactate; Pyruvate; Polypyrrole; Lactobacillus delbruecki sp; L-LACTATE; BODY-COMPOSITION; AMPEROMETRIC BIOSENSORS; MODIFIED ELECTRODES; YEAST-CELLS; OXIDASE; SUPPLEMENTATION; DEHYDROGENASE; FERMENTATION; TISSUE;

D O I：

10.1016/j.foodchem.2014.07.140

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The aim of this study was mainly to develop a microbial biosensor for the simultaneous determination of lactic acid and pyruvic acid. In developing biosensor, lyophilised Lactobacillus delbruecki sp. bacterial cells were immobilised with polypyrrole on a platin electrode surface using electropolymerization method. Lactate concentration was determined based on the differences in amperometric responses at cathodic peak (+0.2 V) of potassium ferricyanide, whereas pyruvate concentration was determined using the differences at anodic peak (+0.1 V). The response of biosensor showed linearity between 0.1 and 1.0 mM for both of two substrates. Optimisation studies were carried out for amount of microorganism, pyrrole concentration, pH and temperature. In the characterisation studies, substrate specificity, interference effect of some substances on the biosensor response, and storage stability were established. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：197 / 202

页数：6

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