The Mechanisms Underlying α-Amanitin Resistance in Drosophila melanogaster: A Microarray Analysis

被引：13

作者：

Mitchell, Chelsea L. ^{[1
]}

Saul, Michael C. ^{[2
]}

Lei, Liang ^{[3
]}

Wei, Hairong ^{[3
]}

Werner, Thomas ^{[1
]}

机构：

[1] Michigan Technol Univ, Dept Biol Sci, Houghton, MI 49931 USA

[2] Univ Wisconsin, Dept Zool, Madison, WI 53706 USA

[3] Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA

来源：

PLOS ONE | 2014年 / 9卷 / 04期

关键词：

GLUTATHIONE-S-TRANSFERASE; VECTOR ANOPHELES-GAMBIAE; INSECTICIDE-RESISTANCE; CYTOCHROME-P450; GENES; DDT RESISTANCE; HETEROLOGOUS EXPRESSION; MYCOPHAGOUS DROSOPHILA; NATURAL-POPULATION; OVER-EXPRESSION; MUSCA-DOMESTICA;

D O I：

10.1371/journal.pone.0093489

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The rapid evolution of toxin resistance in animals has important consequences for the ecology of species and our economy. Pesticide resistance in insects has been a subject of intensive study; however, very little is known about how Drosophila species became resistant to natural toxins with ecological relevance, such as alpha-amanitin that is produced in deadly poisonous mushrooms. Here we performed a microarray study to elucidate the genes, chromosomal loci, molecular functions, biological processes, and cellular components that contribute to the alpha-amanitin resistance phenotype in Drosophila melanogaster. We suggest that toxin entry blockage through the cuticle, phase I and II detoxification, sequestration in lipid particles, and proteolytic cleavage of alpha-amanitin contribute in concert to this quantitative trait. We speculate that the resistance to mushroom toxins in D. melanogaster and perhaps in mycophagous Drosophila species has evolved as cross-resistance to pesticides, other xenobiotic substances, or environmental stress factors.

引用

页数：16

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