A translation repressor element resides in the 3′ untranslated region of human p53 mRNA

被引：35
作者：
Fu, LN
Ma, WL
Benchimol, S
机构：
[1] Princess Margaret Hosp, Ontario Canc Inst, Toronto, ON M5G 2M9, Canada
[2] Univ Toronto, Dept Med Biophys, Toronto, ON M5G 2M9, Canada
来源：
ONCOGENE | 1999年 / 18卷 / 47期
基金：
英国医学研究理事会;
关键词：
p53; translational control; 3 ' UTR;
D O I：
10.1038/sj.onc.1203064
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The 3' untranslated region of human p53 mRNA represses translation both irt vitro and in vivo. Here, we identify a cis-acting 66-nucleotide U-rich sequence in the human p53 mRNA 3' untranslated region that mediates translational repression. Using UV crosslinking, we detect a 40 kDa protein that interacts specifically with the p53 3'UTR containing the repressor element. Enhanced translation of p53 mRNA contributes to the accumulation of p53 protein in cells exposed to gamma-radiation and could be a consequence of relieving the inhibition mediated by the repressor element.
引用
收藏
页码：6419 / 6424
页数：6
相关论文
共 33 条
  • [1] The regulation of the Drosophila msl-2 gene reveals a function for Sex-lethal in translational control
    Bashaw, GJ
    Baker, BS
    [J]. CELL, 1997, 89 (05) : 789 - 798
  • [2] POST-TRANSLATIONAL CONTROL OF RIBOSOMAL-PROTEIN L1 ACCUMULATION IN XENOPUS OOCYTES
    BAUM, EZ
    HYMAN, LE
    WORMINGTON, WM
    [J]. DEVELOPMENTAL BIOLOGY, 1988, 126 (01) : 141 - 149
  • [3] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
  • [4] ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE
    CHIRGWIN, JM
    PRZYBYLA, AE
    MACDONALD, RJ
    RUTTER, WJ
    [J]. BIOCHEMISTRY, 1979, 18 (24) : 5294 - 5299
  • [5] THYMOCYTE APOPTOSIS INDUCED BY P53-DEPENDENT AND INDEPENDENT PATHWAYS
    CLARKE, AR
    PURDIE, CA
    HARRISON, DJ
    MORRIS, RG
    BIRD, CC
    HOOPER, ML
    WYLLIE, AH
    [J]. NATURE, 1993, 362 (6423) : 849 - 852
  • [6] FIREFLY LUCIFERASE GENE - STRUCTURE AND EXPRESSION IN MAMMALIAN-CELLS
    DEWET, JR
    WOOD, KV
    DELUCA, M
    HELINSKI, DR
    SUBRAMANI, S
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (02) : 725 - 737
  • [7] VARIOUS RAT ADULT TISSUES EXPRESS ONLY ONE MAJOR MESSENGER-RNA SPECIES FROM THE GLYCERALDEHYDE-3-PHOSPHATE-DEHYDROGENASE MULTIGENIC FAMILY
    FORT, P
    MARTY, L
    PIECHACZYK, M
    ELSABROUTY, S
    DANI, C
    JEANTEUR, P
    BLANCHARD, JM
    [J]. NUCLEIC ACIDS RESEARCH, 1985, 13 (05) : 1431 - 1442
  • [8] FRITSCHE M, 1993, ONCOGENE, V8, P307
  • [9] Participation of the human p53 3'UTR in translational repression and activation following gamma-irradiation
    Fu, LN
    Benchimol, S
    [J]. EMBO JOURNAL, 1997, 16 (13) : 4117 - 4125
  • [10] Translational regulation of human p53 gene expression
    Fu, LN
    Minden, MD
    Benchimol, S
    [J]. EMBO JOURNAL, 1996, 15 (16) : 4392 - 4401
1234