A translation repressor element resides in the 3′ untranslated region of human p53 mRNA

被引：35
作者：
Fu, LN
Ma, WL
Benchimol, S
机构：
[1] Princess Margaret Hosp, Ontario Canc Inst, Toronto, ON M5G 2M9, Canada
[2] Univ Toronto, Dept Med Biophys, Toronto, ON M5G 2M9, Canada
来源：
ONCOGENE | 1999年 / 18卷 / 47期
基金：
英国医学研究理事会;
关键词：
p53; translational control; 3 ' UTR;
D O I：
10.1038/sj.onc.1203064
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The 3' untranslated region of human p53 mRNA represses translation both irt vitro and in vivo. Here, we identify a cis-acting 66-nucleotide U-rich sequence in the human p53 mRNA 3' untranslated region that mediates translational repression. Using UV crosslinking, we detect a 40 kDa protein that interacts specifically with the p53 3'UTR containing the repressor element. Enhanced translation of p53 mRNA contributes to the accumulation of p53 protein in cells exposed to gamma-radiation and could be a consequence of relieving the inhibition mediated by the repressor element.
引用
收藏
页码：6419 / 6424
页数：6
相关论文
共 33 条
[1]   The regulation of the Drosophila msl-2 gene reveals a function for Sex-lethal in translational control [J].
Bashaw, GJ ;
Baker, BS .
CELL, 1997, 89 (05) :789-798
[2]   POST-TRANSLATIONAL CONTROL OF RIBOSOMAL-PROTEIN L1 ACCUMULATION IN XENOPUS OOCYTES [J].
BAUM, EZ ;
HYMAN, LE ;
WORMINGTON, WM .
DEVELOPMENTAL BIOLOGY, 1988, 126 (01) :141-149
[3]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4]   ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].
CHIRGWIN, JM ;
PRZYBYLA, AE ;
MACDONALD, RJ ;
RUTTER, WJ .
BIOCHEMISTRY, 1979, 18 (24) :5294-5299
[5]   THYMOCYTE APOPTOSIS INDUCED BY P53-DEPENDENT AND INDEPENDENT PATHWAYS [J].
CLARKE, AR ;
PURDIE, CA ;
HARRISON, DJ ;
MORRIS, RG ;
BIRD, CC ;
HOOPER, ML ;
WYLLIE, AH .
NATURE, 1993, 362 (6423) :849-852
[6]   FIREFLY LUCIFERASE GENE - STRUCTURE AND EXPRESSION IN MAMMALIAN-CELLS [J].
DEWET, JR ;
WOOD, KV ;
DELUCA, M ;
HELINSKI, DR ;
SUBRAMANI, S .
MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (02) :725-737
[7]   VARIOUS RAT ADULT TISSUES EXPRESS ONLY ONE MAJOR MESSENGER-RNA SPECIES FROM THE GLYCERALDEHYDE-3-PHOSPHATE-DEHYDROGENASE MULTIGENIC FAMILY [J].
FORT, P ;
MARTY, L ;
PIECHACZYK, M ;
ELSABROUTY, S ;
DANI, C ;
JEANTEUR, P ;
BLANCHARD, JM .
NUCLEIC ACIDS RESEARCH, 1985, 13 (05) :1431-1442
[8]  
FRITSCHE M, 1993, ONCOGENE, V8, P307
[9]   Participation of the human p53 3'UTR in translational repression and activation following gamma-irradiation [J].
Fu, LN ;
Benchimol, S .
EMBO JOURNAL, 1997, 16 (13) :4117-4125
[10]   Translational regulation of human p53 gene expression [J].
Fu, LN ;
Minden, MD ;
Benchimol, S .
EMBO JOURNAL, 1996, 15 (16) :4392-4401
1234