Aging causes morphological alterations in astrocytes and microglia in human substantia nigra pars compacta

被引：106

作者：

Jyothi, H. J. ^{[1
]}

Vidyadhara, D. J. ^{[1
]}

Mahadevan, Anita ^{[2
]}

Philip, Mariamma ^{[3
]}

Parmar, Suresh Kumar ^{[2
]}

Manohari, S. Gowri ^{[1
]}

Shankar, S. K. ^{[2
]}

Raju, Trichur R. ^{[1
]}

Alladi, Phalguni Anand ^{[1
]}

机构：

[1] Natl Inst Mental Hlth & Neurosci, Dept Neurophysiol, Bengaluru 560029, Karnataka, India

[2] Natl Inst Mental Hlth & Neurosci, Dept Neuropathol, Bengaluru 560029, Karnataka, India

[3] Natl Inst Mental Hlth & Neurosci, Dept Biostat, Bengaluru 560029, Karnataka, India

来源：

NEUROBIOLOGY OF AGING | 2015年 / 36卷 / 12期

关键词：

Parkinson's disease; Autopsied human midbrains; Stereology; Morphometry; Densitometry; GFAP; CNPase; Iba1; S100; beta; AGE-RELATED-CHANGES; PARKINSONS-DISEASE; ALPHA-SYNUCLEIN; SUBCELLULAR-DISTRIBUTION; MESSENGER-RNA; GLIAL-CELLS; TNF-ALPHA; NEURONS; ACTIVATION; EXPRESSION;

D O I：

10.1016/j.neurobiolaging.2015.08.024

中图分类号：

R592 [老年病学]; C [社会科学总论];

学科分类号：

03 ; 0303 ; 100203 ;

摘要：

Age being a risk factor for Parkinson's disease, assessment of age-related changes in the human substantia nigra may elucidate its pathogenesis. Increase in Marinesco bodies, alpha-synuclein, free radicals and so forth in the aging nigral neurons are clear indicators of neurodegeneration. Here, we report the glial responses in aging human nigra. The glial numbers were determined on Nissl-stained sections. The expression of glial fibrillary acidic protein, S100 beta, 2', 3'-cyclic nucleotide 3' phosphodiesterase, and Iba1 was assessed on cryosections of autopsied midbrains by immunohistochemistry and densitometry. The glial counts showed a biphasic increase, of which, the first prominent phase from fetal age to birth could be physiological gliogenesis whereas the second one after middle age may reflect mild age-related gliosis. Astrocytic morphology was altered, but glial fibrillary acidic protein expression increased only mildly. Presence of type-4 microglia suggests possibility of neuroinflammation. Mild reduction in 2', 3'-cyclic nucleotide 3' phosphodiesterase-labeled area denotes subtle demyelination. Stable age-related S100 beta expression indicates absence of calcium overload. Against the expected prominent gliosis, subtle age-related morphological alterations in human nigral glia attribute them a participatory role in aging. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：3321 / 3333

页数：13

共 64 条

[1] Postnatal Development of Neurons, Interneurons and Glial Cells in the Substantia Nigra of Mice [J].

Abe, Manami ;

Kimoto, Hiroki ;

Eto, Risa ;

Sasaki, Taeko ;

Kato, Hiroyuki ;

Kasahara, Jiro ;

Araki, Tsutomu .

CELLULAR AND MOLECULAR NEUROBIOLOGY, 2010, 30 (06) :917-928

[2] Actin-binding proteins coronin-1a and IBA-1 are effective microglial markers for immunohistochemistry [J].

Ahmed, Zeshan ;

Shaw, Gerry ;

Sharma, Ved P. ;

Yang, Cui ;

McGowan, Eileen ;

Dickson, Dennis W. .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 2007, 55 (07) :687-700

[3] ABSENCE OF AGE-RELATED CHANGES IN NIGRAL DOPAMINERGIC NEURONS OF ASIAN INDIANS: RELEVANCE TO LOWER INCIDENCE OF PARKINSON'S DISEASE [J].

Alladi, P. A. ;

Mahadevan, A. ;

Yasha, T. C. ;

Raju, T. R. ;

Shankar, S. K. ;

Muthane, U. .

NEUROSCIENCE, 2009, 159 (01) :236-245

[4] Ageing enhances α-synuclein, ubiquitin and endoplasmic reticular stress protein expression in the nigral neurons of Asian Indians [J].

Alladi, Phalguni Anand ;

Mahadevan, Anita ;

Vijayalakshmi, K. ;

Muthane, Uday ;

Shankar, S. K. ;

Raju, T. R. .

NEUROCHEMISTRY INTERNATIONAL, 2010, 57 (05) :530-539

[5] Expression of GDNF receptors GFRα1 and RET is preserved in substantia nigra pars compacta of aging Asian Indians [J].

Alladi, Phalguni Anand ;

Mahadevan, Anita ;

Shankar, S. M. ;

Raju, T. R. ;

Muthane, Uday .

JOURNAL OF CHEMICAL NEUROANATOMY, 2010, 40 (01) :43-52

[6] Evidence of oligodendrogliosis in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinsonism [J].

Annese, V. ;

Barcia, C. ;

Ros-Bernal, F. ;

Gomez, A. ;

Ros, C. M. ;

De Pablos, V. ;

Fernandez-Villalba, E. ;

De Stefano, M. E. ;

Herrero, M-T .

NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY, 2013, 39 (02) :132-143

[7] Glial pathology but absence of apoptotic nigral neurons in long-standing Parkinson's disease [J].

Banati, RB ;

Daniel, SE ;

Blunt, SB .

MOVEMENT DISORDERS, 1998, 13 (02) :221-227

[8] IFN-γ signaling, with the synergistic contribution of TNF-α, mediates cell specific microglial and astroglial activation in experimental models of Parkinson's disease [J].

Barcia, C. ;

Ros, C. M. ;

Annese, V. ;

Gomez, A. ;

Ros-Bernal, F. ;

Aguado-Yera, D. ;

Martinez-Pagan, M. E. ;

de Pablos, V. ;

Fernandez-Villalba, E. ;

Herrero, M. T. .

CELL DEATH & DISEASE, 2011, 2 :e142-e142

[9] Increase of Secondary Processes of Microglial and Astroglial Cells After MPTP-Induced Degeneration in Substantia Nigra Pars Compacta of Non Human Primates [J].

Barcia, Carlos ;

Ros, Carmen M. ;

Carrillo, Maria A. ;

Ros, Francisco ;

Gomez, Aurora ;

de Pablos, Vicente ;

Bautista-Hernandez, Victor ;

Sanchez-Bahillo, Angel ;

Fernandez Villalba, Emiliano ;

Herrero, Maria-Trinidad .

JOURNAL OF NEURAL TRANSMISSION-SUPPLEMENT, 2009, (73) :253-258

[10] The Mystery and Magic of Glia: A Perspective on Their Roles in Health and Disease [J].

Barres, Ben A. .

NEURON, 2008, 60 (03) :430-440

← 1 2 3 4 5 6 7 →