Study on the interaction of copper-zinc superoxide dismutase with cobalt(II)-histidine by spectral analysis -: II.: Effect of amount of external added Co(His)n, phosphate

The effect of changing the amount of external added Co(His), and phosphate on the interaction of Cu2Zn2SOD with organic metal compound (Cobalt(II)-Histidine) have been studied by means of ICP,VIS and measurement of enzyme activity. It has found that in aqueous solution, there exists a direct interaction of the metal ions of the active center in the metalloenzyme (Cu2Zn2SOD) with external added Co(His)(n). As a result, part of the metal ions in metalloenzyme were replaced and the corresponding metalloenzyme derivatives (Co(II)-substituted derivatives of SOD) were produced and the catalytic activity of enzyme were affected. It has also studied the intensity of interactions in different molar ratios (Cobalt(II)-Histidine) and of the presence of phosphate and got some results under the effect of corresponding factors.