Study on the interaction of copper-zinc superoxide dismutase with cobalt(II)-histidine by spectral analysis -: II.: Effect of amount of external added Co(His)n, phosphate

被引:0
作者
Zheng, XF [1 ]
Hu, JH
Cheng, GB
Xu, YT
Zhao, YK
Liu, MH
机构
[1] Dalian Univ Technol, Dept Bioengn, Dalian 116012, Peoples R China
[2] Acad Sinica, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
[3] Dalian Univ, Dept Chem & Chem Engn, Dalian 116622, Peoples R China
[4] Liaoning Normal Univ, Expt Ctr, Dalian 116021, Peoples R China
关键词
Cu2Zn2SOD; Co(His)(n); interaction;
D O I
暂无
中图分类号
O433 [光谱学];
学科分类号
0703 ; 070302 ;
摘要
The effect of changing the amount of external added Co(His), and phosphate on the interaction of Cu2Zn2SOD with organic metal compound (Cobalt(II)-Histidine) have been studied by means of ICP,VIS and measurement of enzyme activity. It has found that in aqueous solution, there exists a direct interaction of the metal ions of the active center in the metalloenzyme (Cu2Zn2SOD) with external added Co(His)(n). As a result, part of the metal ions in metalloenzyme were replaced and the corresponding metalloenzyme derivatives (Co(II)-substituted derivatives of SOD) were produced and the catalytic activity of enzyme were affected. It has also studied the intensity of interactions in different molar ratios (Cobalt(II)-Histidine) and of the presence of phosphate and got some results under the effect of corresponding factors.
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页码:798 / 802
页数:5
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