Protective Role of Probiotic Supplements in Hepatic Steatosis: A Rat Model Study

被引:35
作者
Azarang, Aein [1 ,2 ]
Farshad, Omid [1 ,2 ]
Ommati, Mohammad Mehdi [3 ]
Jamshidzadeh, Akram [4 ]
Heidari, Reza [1 ,4 ]
Abootalebi, Seyedeh Narjes [5 ]
Gholami, Ahmad [6 ]
机构
[1] Shiraz Univ Med Sci, Pharmaceut Sci Res Ctr, Shiraz, Iran
[2] Shiraz Univ Med Sci, Sch Pharm, Dept Pharmaceut Biotechnol, Shiraz, Iran
[3] Shanxi Agr Univ, Coll Life Sci, Dept Bioinformat, Taigu, Peoples R China
[4] Shiraz Univ Med Sci, Sch Pharm, Dept Pharmacol & Toxicol, Shiraz, Iran
[5] Shiraz Univ Med Sci, Sch Med, Div Intens Care Unit, Dept Pediat, Shiraz, Iran
[6] Shiraz Univ Med Sci, Biotechnol Res Ctr, Shiraz, Iran
关键词
FATTY LIVER-DISEASE; GUT MICROBIOTA; LACTOBACILLUS-CASEI; OXIDATIVE STRESS; ANIMAL-MODELS; FRUCTOSE; ACID; COMBINATION; METABOLISM; DISORDERS;
D O I
10.1155/2020/5487659
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background. Treating nonalcoholic fatty liver disease (NAFLD) is considered one of the public health priorities in the past decade. So far, probiotics have represented promising results in controlling the signs and symptoms of NAFLD. However, attempts to find the ideal probiotic strain are still ongoing. The present study is designed to find the best strain amongst suitable probiotic strains according to their ability to ameliorate histopathological and oxidative stress biomarkers in hepatic steatosis-induced rats. Methods. Initially, four probiotics species, including Lactobacillus (L.) acidophilus, L. casei, L. reuteri, and Bacillus coagulans, were cultured and prepared as a lyophilized powder for animals. The experiment lasted for fifty days. Initially, hepatic steatosis was induced by excessive ingestion of D-fructose in rats for eight weeks, followed by eight weeks of administering probiotics and D-fructose concurrently. Forty-two six-week-old male rats were alienated to different groups and were supplemented with different probiotics (1*109 CFU in 500 mL drinking water). After eight weeks, blood and liver samples were taken for further evaluation, and plasma and oxidative stress markers corresponding to liver injuries were examined. Results. Administration of probiotics over eight weeks reversed hepatic and blood triglyceride concentration and blood glucose levels. Also, probiotics significantly suppressed markers of oxidative stress in the liver tissue. Conclusions. Although some of the single probiotic formulations were able to mitigate oxidative stress markers, mixtures of probiotics significantly ameliorated more symptoms in the NAFLD animals. This enhanced effect might be due to probiotics' cumulative potential to maintain oxidative stress and deliver improved lipid profiles, liver function markers, and inflammatory markers.
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页数:15
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