Reduced Insulin/Insulin-like Growth Factor-1 Signaling and Dietary Restriction Inhibit Translation but Preserve Muscle Mass in Caenorhabditis elegans

被引:66
作者
Depuydt, Geert [1 ]
Xie, Fang [2 ,3 ]
Petyuk, Vladislav A. [2 ,3 ]
Shanmugam, Nilesh [1 ]
Smolders, Arne [1 ]
Dhondt, Ineke [1 ]
Brewer, Heather M. [2 ,3 ]
Camp, David G. [2 ,3 ]
Smith, Richard D. [2 ,3 ]
Braeckman, Bart P. [1 ]
机构
[1] Univ Ghent, Dept Biol, B-9000 Ghent, Belgium
[2] Pacific NW Natl Lab, Biol Sci Div, Richland, WA 99352 USA
[3] Pacific NW Natl Lab, Environm Mol Sci Lab, Richland, WA 99352 USA
关键词
LIFE-SPAN EXTENSION; MESSENGER-RNA TRANSLATION; CHAIN AMINO-ACIDS; C-ELEGANS; GENE-EXPRESSION; PROTEIN-SYNTHESIS; INDUCED LONGEVITY; DAUER LARVAE; SACCHAROMYCES-CEREVISIAE; LIQUID-CHROMATOGRAPHY;
D O I
10.1074/mcp.M113.027383
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Reduced signaling through the C. elegans insulin/insulinlike growth factor-1-like tyrosine kinase receptor daf-2 and dietary restriction via bacterial dilution are two wellcharacterized lifespan-extending interventions that operate in parallel or through (partially) independent mechanisms. Using accurate mass and time tag LC-MS/MS quantitative proteomics, we detected that the abundance of a large number of ribosomal subunits is decreased in response to dietary restriction, as well as in the daf-2(e1370) insulin/insulin-like growth factor-1-receptor mutant. In addition, general protein synthesis levels in these long-lived worms are repressed. Surprisingly, ribosomal transcript levels were not correlated to actual protein abundance, suggesting that post-transcriptional regulation determines ribosome content. Proteomics also revealed the increased presence of many structural muscle cell components in long-lived worms, which appeared to result from the prioritized preservation of muscle cell volume in nutrient-poor conditions or low insulin-like signaling. Activation of DAF-16, but not diet restriction, stimulates mRNA expression of muscle-related genes to prevent muscle atrophy. Important daf-2-specific proteome changes include overexpression of aerobic metabolism enzymes and general activation of stress-responsive and immune defense systems, whereas the increased abundance of many protein subunits of the proteasome core complex is a dietaryrestriction-specific characteristic. Molecular & Cellular Proteomics 12: 10.1074/mcp.M113.027383, 3624-3639, 2013. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.
引用
收藏
页码:3624 / 3639
页数:16
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