Glial cell line-derived neurotrophic factor induced the differentiation of amniotic fluid-derived stem cells into vascular endothelial-like cells in vitro

被引:15
作者
Zhang, Ruyu [1 ,2 ]
Lu, Ying [1 ,2 ]
Li, Ju [3 ]
Wang, Jia [1 ]
Liu, Caixia [1 ,2 ]
Gao, Fang [4 ]
Sun, Dong [1 ,2 ]
机构
[1] Affiliated Hosp Xuzhou, Coll Med, Dept Nephrol, 99 West Huai Hai Rd, Xuzhou 221002, Jiangsu, Peoples R China
[2] Xuzhou Med Coll, Dept Internal Med & Diagnost, Xuzhou 221002, Peoples R China
[3] Nanjing Med Univ, Huaian Peoples Hosp 1, Huaian 223300, Jiangsu, Peoples R China
[4] Affiliated Hosp, Xuzhou Med Coll, Dept Anesthesiol, Xuzhou 221002, Peoples R China
基金
中国国家自然科学基金;
关键词
Amniotic fluid-derived stem cells; Glial cell line-derived neurotrophic factor; Transfection; Endothelial-like cells; GROWTH-FACTOR; FACTOR GDNF; ANGIOGENESIS; NEURONS; VASCULOGENESIS; PROLIFERATION; EXPRESSION; SURVIVAL; VIVO;
D O I
10.1007/s10735-015-9649-9
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Amniotic fluid-derived stem cells (AFSCs) are a novel source of stem cells that are isolated and cultured from second trimester amniocentesis. Glial cell line-derived neurotrophic factor (GDNF) acts as a tissue morphogen and regulates stem cell proliferation and differentiation. This study investigated the effect of an adenovirus-mediated GDNF gene, which was engineered into AFSCs, on the cells' biological properties and whether GDNF in combination with AFSCs can be directionally differentiated into vascular endothelial-like cells in vitro. AFSCs were isolated and cultured using the plastic adherence method in vitro and identified by the transcription factor Oct-4, which is the primary marker of pluripotent stem cells. AFSCs were efficiently transfected by a GFP-labeled plasmid system of an adenovirus vector carrying the GDNF gene (Ad-GDNF-GFP). Transfected AFSCs stably expressed GDNF. Transfected AFSCs were cultured in endothelial growth medium-2 containing vascular endothelial growth factor. After 1 week, AFSCs were positive for von Willebrand factor (vWF) and CD31, which are markers of endothelial cells, and the recombinant GDNF group was significantly higher than undifferentiated controls and the GFP only group. These results demonstrated that AFSCs differentiated into vascular endothelial-like cells in vitro, and recombinant GDNF promoted differentiation. The differentiation-induced AFSCs may be used as seed cells to provide a new manner of cell and gene therapies for transplantation into the vascular injury site to promote angiogenesis.
引用
收藏
页码:9 / 19
页数:11
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