Exploiting algal NADPH oxidase for biophotovoltaic energy

被引:38
作者
Anderson, Alexander [1 ,5 ]
Laohavisit, Anuphon [1 ,6 ]
Blaby, Ian K. [2 ]
Bombelli, Paolo [3 ]
Howe, Christopher J. [3 ]
Merchant, Sabeeha S. [4 ]
Davies, Julia M. [1 ]
Smith, Alison G. [1 ]
机构
[1] Univ Cambridge, Dept Plant Sci, Cambridge, England
[2] Univ Calif Los Angeles, Dept Chem & Biochem, 405 Hilgard Ave, Los Angeles, CA 90024 USA
[3] Univ Cambridge, Dept Biochem, Tennis Court Rd, Cambridge CB2 1QW, England
[4] Univ Calif Los Angeles, Inst Genom & Prote, Los Angeles, CA USA
[5] Univ Cambridge, Dept Physiol Dev & Neurosci, Cambridge CB2 3EG, England
[6] RIKEN, Ctr Sustainable Resource Sci, 1-7-22 Suehiro, Yokohama, Kanagawa 2300045, Japan
基金
美国国家卫生研究院; 英国工程与自然科学研究理事会;
关键词
alga; biophotovoltaic; Chlamydomonas; energy; NADPH oxidase; PHYTOPLANKTON CHATTONELLA-MARINA; CHLAMYDOMONAS-REINHARDTII; SUPEROXIDE-DISMUTASE; OXIDATIVE BURST; CELL-GROWTH; HOMOLOGS; PROVIDE; SYSTEM; ANION; WALL;
D O I
10.1111/pbi.12332
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Photosynthetic microbes exhibit light-dependent electron export across the cell membrane, which can generate electricity in biological photovoltaic (BPV) devices. How electrons are exported remains to be determined; the identification of mechanisms would help selection or generation of photosynthetic microbes capable of enhanced electrical output. We show that plasma membrane NADPH oxidase activity is a significant component of light-dependent generation of electricity by the unicellular green alga Chlamydomonas reinhardtii. NADPH oxidases export electrons across the plasma membrane to form superoxide anion from oxygen. The C. reinhardtii mutant lacking the NADPH oxidase encoded by RBO1 is impaired in both extracellular superoxide anion production and current generation in a BPV device. Complementation with the wild-type gene restores both capacities, demonstrating the role of the enzyme in electron export. Monitoring light-dependent extracellular superoxide production with a colorimetric assay is shown to be an effective way of screening for electrogenic potential of candidate algal strains. The results show that algal NADPH oxidases are important for superoxide anion production and open avenues for optimizing the biological component of these devices.
引用
收藏
页码:22 / 28
页数:7
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