ATR-FTIR spectroscopy: Its advantages and limitations

被引:0
|
作者
Grdadolnik, J [1 ]
机构
[1] Natl Inst Chem, SI-1000 Ljubljana, Slovenia
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中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
In this article the main advantages and limitations of the ATR(.) technique are presented. As examples, the ATR spectra of liquid bulk water and a water solution of protein bovine serum albumin are used. The main differences between the normal transmission spectroscopy and ATR are pointed out. Also shown is the way to calculate the optical constant, using the CIRCLE(TM) ATR liquid cell, and from optical constants how to calculate epsilon' and epsilon" as well as pure absorbance spectrum, which is fully comparable with the normal transmission ones.
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页码:631 / 642
页数:12
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