Oligonucleotide-templated reactions based on Peptide Nucleic Acid (PNA) probes: Concept and biomedical applications

被引：17

作者：

Choi, Youngeun ^{[1
]}

Metcalf, Gavin ^{[1
]}

Sleiman, Mazen Haj ^{[1
]}

Vair-Turnbull, Douglas ^{[1
]}

Ladame, Sylvain ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Bioengn, London SW7 2AZ, England

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2014年 / 22卷 / 16期

关键词：

Peptide Nucleic Acids; Oligonucleotide templated reaction; Biomarkers; Cell-free nucleic acids; Cancer; CYANINE DYES; LIVE CELLS; DNA; RNA; AMPLIFICATION; FLUOROPHORE; ACTIVATION; REDUCTION; BIOMARKER; HAIRPIN;

D O I：

10.1016/j.bmc.2014.05.071

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sensing technologies based on Peptide Nucleic Acids (PNAs) and oligonucleotide-templated chemistry are perfectly suited for biomedical applications (e.g., diagnosis, prognosis and stratification of diseases) and could compete well with more traditional amplification technologies using expensive dual-labelled oligonucleotide probes. PNAs can be easily synthesised and functionalised, are more stable and are more responsive to point-mutations than their DNA counterpart. For these reasons, fluorogenic PNAs represent an interesting alternative to DNA-based molecular beacons for sensing applications in a cell-free environment, where cellular uptake is not required. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：4395 / 4398

页数：4

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