The HPLC stereoselective resolution of N-2,4-dinitrophenylated (DNP) amine-containing enantiomers on teicoplanin bonded phase using the methanol-based mobile phase

A variety of compounds containing an amine group (i.e., amino acids and amino alcohols) are fast and much better than baseline enantioresolved on a teicoplanin bonded chiral phase using the methanol-based mobile phase after their pre-column derivatization with 2,4-dinitrofluorobenzene (DNFB) in alkaline medium. The resolution is found relatively insensitive to the structural variations of the analyte and is enhanced as the stereogenic center of analyte is close to the nitro group substituted aromatic moiety of the tagging reagent for stronger pi-pi interaction. The resolution is either not observed or unsatisfactory using the acetonitrile-based mobile phase.