Type I fimbriae subunit fimA enhances Escherichia coli biofilm formation but affects L-threonine carbon distribution

被引：8

作者：

Liu, Qingguo ^{[1
,2
]}

Zhu, Jiaqing ^{[1
]}

Liu, Na ^{[1
]}

Sun, Wenjun ^{[1
]}

Yu, Bin ^{[1
]}

Niu, Huanqing ^{[1
]}

Liu, Dong ^{[1
]}

Ouyang, Pingkai ^{[1
]}

Ying, Hanjie ^{[1
]}

Chen, Yong ^{[1
]}

Zhao, Gulin ^{[1
]}

Chen, Tianpeng ^{[1
]}

机构：

[1] Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, State Key Lab Mat Oriented Chem Engn, Nanjing, Peoples R China

[2] Nanjing Hitech Biol Technol Res Inst Co Ltd, Nanjing, Peoples R China

来源：

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY | 2022年 / 10卷

基金：

中国国家自然科学基金; 国家重点研发计划;

关键词：

Escherichia coli; fimA gene; biofilm; L-threonine carbon distribution; transcriptomic analysis; COLI; AUTOINDUCER-2; EXPRESSION; MOTILITY; ADHESION; PATHWAY; MUTANT; LUXS;

D O I：

10.3389/fbioe.2022.904636

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The biofilm (BF) provides favorable growth conditions to cells, which has been exploited in the field of industrial biotechnology. Based on our previous research works on type I fimbriae for the biosynthesis of L-threonine (LT) in Escherichia coli, in this study, a fimA-overexpressing strain was engineered, which improved BF formation under industrial fermentation conditions. The morphological observation and characterization of BF formation were conducted to verify the function of the subunit FimA. However, it was not suitable for repeated-batch immobilized fermentation as the LT titer was not elevated significantly. The underlying molecular mechanisms of BF formation and the LT carbon flux were explored by transcriptomic analysis. The results showed that fimA regulated E. coli BF formation but affected LT carbon distribution. This study will stimulate thoughts about how the fimbriae gene regulated biofilms and amino acid excretion and will bring some consideration and provide a reference for the development of BF-based biomanufacturing processes in E. coli.

引用

页数：11

共 32 条

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