Sensitive and rapid detection of blaNDM-1 in clinical samples by isothermal cross-priming amplification

被引：10

作者：

Ke, Yuehua ^{[1
]}

Wang, Yufei ^{[1
]}

Wang, Zhoujia ^{[1
]}

Du, Xinying ^{[1
]}

Huang, Liuyu ^{[1
]}

Chen, Zeliang ^{[1
]}

机构：

[1] Acad Mil Med Sci, Inst Dis Control & Prevent, Beijing 100071, Peoples R China

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2013年 / 95卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Cross priming amplification; bla(NDM-1); Isothermal amplification; EMERGENCE; MECHANISM; GENE;

D O I：

10.1016/j.mimet.2013.07.017

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A highly sensitive, specific diagnostic assay for detection of bla(NDM-1) based on cross priming amplification (CPA) was developed. The sensitivity ranged from 2.5 to 25 copies per reaction for different clinical samples. The highly and sensitive detection of bla(NDM-1), in clinical samples highlighted the potential clinical applications of CPA (C) 2013 Published by Elsevier B.V.

引用

页码：215 / 217

页数：3

共 7 条

[1] Emergence of NDM-1-producing Acinetobacter baumannii in China [J].

Chen, Yan ;

Zhou, Zhihui ;

Jiang, Yan ;

Yu, Yunsong .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2011, 66 (06) :1255-1259

[2] Real-time PCR assay and a synthetic positive control for the rapid and sensitive detection of the emerging resistance gene New Delhi Metallo-β-lactamase-1 (blaNDM-1) [J].

Kruettgen, Alexander ;

Razavi, Soheila ;

Imoehl, Matthias ;

Ritter, Klaus .

MEDICAL MICROBIOLOGY AND IMMUNOLOGY, 2011, 200 (02) :137-141

[3] Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study [J].

Kumarasamy, Karthikeyan K. ;

Toleman, Mark A. ;

Walsh, Timothy R. ;

Bagaria, Jay ;

Butt, Fafhana ;

Balakrishnan, Ravikumar ;

Chaudhary, Uma ;

Doumith, Michel ;

Giske, Christian G. ;

Irfan, Seema ;

Krishnan, Padma ;

Kumar, Anil V. ;

Maharjan, Sunil ;

Mushtaq, Shazad ;

Noorie, Tabassum ;

Paterson, David L. ;

Pearson, Andrew ;

Perry, Claire ;

Pike, Rachel ;

Rao, Bhargavi ;

Ray, Ujjwayini ;

Sarma, Jayanta B. ;

Sharma, Madhu ;

Sheridan, Elizabeth ;

Thirunarayan, Mandayam A. ;

Turton, Jane ;

Upadhyay, Supriya ;

Warner, Marina ;

Welfare, William ;

Livermore, David M. ;

Woodford, Neil .

LANCET INFECTIOUS DISEASES, 2010, 10 (09) :597-602

[4] Sensitive and Rapid Detection of the New Delhi Metallo-Beta-Lactamase Gene by Loop-Mediated Isothermal Amplification [J].

Liu, Wei ;

Zou, Dayang ;

Li, Yan ;

Wang, Xuesong ;

He, Xiang ;

Wei, Xiao ;

Shao, Changlin ;

Li, Xuelian ;

Shang, Wei ;

Yu, Kaitao ;

Liu, Dawei ;

Li, Yunmei ;

Guo, Jing ;

Yin, Zhitao ;

Yuan, Jing .

JOURNAL OF CLINICAL MICROBIOLOGY, 2012, 50 (05) :1580-1585

[5] Evaluation of a DNA Microarray (Check-MDR CT102) for Rapid Detection of TEM, SHV, and CTX-M Extended-Spectrum β-Lactamases and of KPC, OXA-48, VIM, IMP, and NDM-1 Carbapenemases [J].

Naas, Thierry ;

Cuzon, Gaelle ;

Bogaerts, Pierre ;

Glupczynski, Youri ;

Nordmann, Patrice .

JOURNAL OF CLINICAL MICROBIOLOGY, 2011, 49 (04) :1608-1613

[6] A Loop-Mediated Isothermal Amplification Method for Rapid Detection of NDM-1 Gene [J].

Qi, Jing ;

Du, Yijun ;

Zhu, Xiaoling ;

Bai, Hua ;

Luo, Yanbo ;

Liu, Yuqing .

MICROBIAL DRUG RESISTANCE, 2012, 18 (04) :359-363

[7]

Xu GL, 2012, SCI REP-UK, V2, DOI [10.1038/srep00246, 10.1038/srep00986]

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