Lung Mast Cells Have a High Constitutive Expression of Carboxypeptidase A3 mRNA That Is Independent from Granule-Stored CPA3

被引:21
作者
Siddhuraj, Premkumar [1 ]
Clausson, Carl-Magnus [1 ]
Sanden, Caroline [1 ,2 ]
Alyamani, Manar [1 ]
Kadivar, Mohammad [1 ]
Marsal, Jan [3 ]
Wallengren, Joanna [4 ]
Bjermer, Leif [5 ]
Erjefalt, Jonas S. [1 ,5 ]
机构
[1] Lund Univ, Dept Expt Med Sci, S-22184 Lund, Sweden
[2] Medetect AB, S-22381 Lund, Sweden
[3] Lund Univ, Skane Univ Hosp, Dept Gastroenterol, S-22185 Lund, Sweden
[4] Lund Univ, Skane Univ Hosp, Dept Dermatol, S-22185 Lund, Sweden
[5] Lund Univ, Skane Univ Hosp, Dept Allergol & Resp Med, S-22185 Lund, Sweden
关键词
mast cells; carboxypeptidase A3; lung; gut; skin; chymase; tryptase; mRNA;
D O I
10.3390/cells10020309
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The mast cell granule metalloprotease CPA3 is proposed to have important tissue homeostatic functions. However, the basal CPA3 mRNA and protein expression among mast cell populations has remained poorly investigated. Using a novel histology-based methodology that yields quantitative data on mRNA and protein expression at a single-cell level, the present study maps CPA3 mRNA and protein throughout the MCT and MCTC populations in healthy skin, gut and lung tissues. MCTC cells had both a higher frequency of CPA3 protein-containing cells and a higher protein-staining intensity than the MCT population. Among the tissues, skin MCs had highest CPA3 protein intensity. The expression pattern at the mRNA level was reversed. Lung mast cells had the highest mean CPA3 mRNA staining. Intriguingly, the large alveolar MCT population, that lack CPA3 protein, had uniquely high CPA3 mRNA intensity. A broader multi-tissue RNA analysis confirmed the uniquely high CPA3 mRNA quantities in the lung and corroborated the dissociation between chymase and CPA3 at the mRNA level. Taken together, our novel data suggest a hitherto underestimated contribution of mucosal-like MCT to baseline CPA3 mRNA production. The functional consequence of this high constitutive expression now reveals an important area for further research.
引用
收藏
页码:1 / 19
页数:17
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