The sequence and phylogenesis of the α-globin genes of Barbary sheep (Ammotragus lervia), goat (Capra hircus), European mouflon (Ovis aries musimon) and Cyprus mouflon (Ovis aries ophion)

被引：5
作者：
Pirastru, Monica [1 ,2 ]
Multineddu, Chiara [1 ,2 ]
Mereu, Paolo [1 ,2 ]
Sannai, Mara [1 ,2 ]
el Sherbini, El Said
Hadjisterkotis, Eleftherios [3 ]
Nahlik, Andras [4 ]
Franceschi, Paul
Manca, Laura [1 ,2 ]
Masala, Bruno [1 ,2 ]
机构：
[1] Univ Sassari, Dipartimento Sci Fisiol Biochim & Cellulari, I-07100 Sassari, Italy
[2] Univ Sassari, Ctr Biotechnol Dev & Biodivers Res, I-07100 Sassari, Italy
[3] Minist Interior, Nicosia, Cyprus
[4] Univ W Hungary, Inst Wildlife Management, Sopron, Hungary
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS | 2009年 / 4卷 / 03期
关键词：
Ammotragus lervia; Capra hircus; Ovis aries musimon; Ovis aries ophion; alpha-globin gene; alpha-globin; Sequencing; Phylogeny; PERFORMANCE LIQUID-CHROMATOGRAPHY; CONCERTED EVOLUTION; HEMOGLOBIN; CHAINS; DUPLICATION; ADULT; DNA; CONVERSION; EXPRESSION; HOMOLOGY;
D O I：
10.1016/j.cbd.2009.02.002
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In order to investigate the polymorphism of alpha-globin chain of hemoglobin amongst caprines, the linked (I)alpha and (II)alpha globin genes of Barbary sheep (Ammotragus lervia), goat (Capra hircus), European mouflon (Ovis aries musimon), and Cyprus mouflon (Ovis aries ophion) were completely sequenced, including the 5' and 3' untranslated regions. European and Cyprus mouflons, which do not show polymorphic alpha globin chains, had almost identical alpha globin genes, whereas Barbary sheep exhibit two different chains encoded by two nonallelic genes. Four different alpha genes were observed and sequenced in goat, validating previous observations of the existence of allelic and nonallelic polymorphism. As in other vertebrates, interchromosomal gene conversion appears to be responsible for such polymorphism. Evaluation of nucleotide sequences at the level of molecular evolution of the (I)alpha-globin gene family in the caprine taxa suggests a closer relationship between the genus Ammotragus and Capra. Molecular clock estimates suggest sheep-mouflon, goat-aoudad, and ancestor-caprine divergences of 2.8, 5.7, and 7.1 MYBP, respectively. (C) 2009 Elsevier Inc. All rights reserved.
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收藏
页码：168 / 173
页数：6
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