Immobilization of Trichoderma harzianum α-Amylase on Treated Wool: Optimization and Characterization

被引：48

作者：

Mohamed, Saleh A. ^{[1
,2
]}

Khan, Jalaluddin A. ^{[1
]}

Al-Bar, Omar A. M. ^{[1
]}

El-Shishtawy, Reda M. ^{[3
,4
]}

机构：

[1] King Abdulaziz Univ, Fac Sci, Dept Biochem, Jeddah 21589, Saudi Arabia

[2] Natl Res Ctr, Dept Mol Biol, Cairo 12622, Egypt

[3] King Abdulaziz Univ, Fac Sci, Dept Chem, Jeddah 21589, Saudi Arabia

[4] Natl Res Ctr, Text Res Div, Dyeing Printing & Text Auxiliaries Dept, Cairo 12622, Egypt

来源：

MOLECULES | 2014年 / 19卷 / 06期

关键词：

Trichoderma harzianum; alpha-amylase; immobilized enzyme; optimization; COVALENT IMMOBILIZATION; ENZYME STABILIZATION; BEADS; MICROSPHERES; IMPROVEMENT; STRATEGIES; STABILITY; MEMBRANES; LIGAND;

D O I：

10.3390/molecules19068027

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

alpha-Amylase from Trichoderma harzianum was covalently immobilized on activated wool by cyanuric chloride. Immobilized alpha-amylase exhibited 75% of its initial activity after 10 runs. The soluble and immobilized alpha-amylases exhibited maximum activity at pH values 6.0 and 6.5, respectively. The immobilized enzyme was more thermally stable than the soluble one. Various substrates were hydrolyzed by immobilized alpha-amylase with high efficiencies compared to those of soluble alpha-amylase. The inhibition of the immobilized alpha-amylase by metal ions was low as compared with soluble enzyme. On the basis of the results obtained, immobilized alpha-amylase could be employed in the saccharification of starch processing.

引用

页码：8027 / 8038

页数：12

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