Reducing cryoEM file storage using lossy image formats

被引：8

作者：

Eng, Edward T. ^{[1
]}

Kopylov, Mykhailo ^{[1
]}

Negro, Carl J. ^{[1
]}

Dallaykan, Sarkis ^{[1
]}

Rice, William J. ^{[1
]}

Jordan, Kelsey D. ^{[1
]}

Kelley, Kotaro ^{[1
]}

Carragher, Bridget ^{[1
,2
]}

Potter, Clinton S. ^{[1
,2
]}

机构：

[1] New York Struct Biol Ctr, Simons Electron Microscopy Ctr, 89 Convent Ave, New York, NY 10027 USA

[2] Columbia Univ, Dept Biochem & Mol Biophys, 630 W 168th St, New York, NY 10032 USA

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 2019年 / 207卷 / 01期

关键词：

Cryo electron microscopy (cryoEM); File format; Image data; Data archiving; Structural biology; ELECTRON; MICROSCOPY;

D O I：

10.1016/j.jsb.2019.04.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent advances in instrumentation and software for cryoEM have increased the applicability and utility of this method. High levels of automation and faster data acquisition rates require hard decisions to be made regarding data retention. Here we investigate the efficacy of data compression applied to aligned summed movie files. Surprisingly, these images can be compressed using a standard lossy method that reduces file storage by 90-95% and yet can still be processed to provide sub-2 angstrom reconstructed maps. We do not advocate this as an archival method, but it may provide a useful means for retaining images as an historical record, especially at large facilities.

引用

页码：49 / 55

页数：7

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