Discrimination of viable and dead microbial materials with Fourier transform infrared spectroscopy in 3-5 micrometers

被引：18

作者：

Gu, Youlin ^{[1
,2
]}

Hu, Yihua ^{[1
,2
]}

Zhao, Xinying ^{[1
,2
]}

Chen, Xi ^{[1
,2
]}

Wang, Peng ^{[3
]}

Zheng, Zhiming ^{[3
]}

机构：

[1] Natl Univ Def Technol, State Key Lab Pulsed Power Laser Technol, Hefei 230037, Anhui, Peoples R China

[2] Natl Univ Def Technol, Anhui Prov Key Lab Elect Restrict, Hefei 230037, Anhui, Peoples R China

[3] Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab Ion Beam Bioengn, Hefei 230001, Anhui, Peoples R China

来源：

OPTICS EXPRESS | 2018年 / 26卷 / 12期

基金：

中国国家自然科学基金;

关键词：

LISTERIA-MONOCYTOGENES CELLS; REAL-TIME PCR; PROPIDIUM MONOAZIDE; ETHIDIUM MONOAZIDE; MICROSCOPY; SCATTERING; PARTICLES; AEROSOLS;

D O I：

10.1364/OE.26.015842

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We present a method to show that average mass extinction coefficient of microbes evaluated via Lorenz-Mie theory can be used to discriminate between viable and dead microbes. Reflectance of viable and dead self-cultured fungal spores and mycelia were measured by the Fourier transform infrared spectroscopy. Complex refractive indices and mass extinction coefficient of viable and dead fungal spores and mycelia were obtained in terms of Kramers-Kronig (KK) relation and Lorenz-Mie theory respectively. Smoke box experimental system was built to validate the effectiveness of the method. The results show that viable and dead fungal spores and mycelia via average mass extinction coefficients can be distinguished. The method can be used to discriminate the bioactivity of microbes and has potential applications in identification, detection, and optical characteristics of viable and dead microbial materials. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

引用

页码：15842 / 15850

页数：9

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