Silver percutaneous absorption after exposure to silver nanoparticles: A comparison study of three human skin graft samples used for clinical applications

被引:34
作者
Bianco, C. [1 ]
Adami, G. [1 ]
Crosera, M. [1 ]
Larese, F. [2 ]
Casarin, S. [3 ]
Castagnoli, C. [3 ]
Stella, M. [3 ]
Maina, G. [4 ,5 ]
机构
[1] Univ Trieste, Dept Chem & Pharmaceut Sci, I-34127 Trieste, Italy
[2] Univ Trieste, Inst Occupat Med, Dept Med Sci, I-34129 Trieste, Italy
[3] CTO Hosp, Dept Plast Surg, I-10126 Turin, Italy
[4] CTO Hosp, Burn Unit Skin Bank, I-10126 Turin, Italy
[5] Univ Turin, Dept Clin & Biol Sci, I-10043 Turin, Italy
关键词
Silver nanoparticles; Skin; Percutaneous absorption; Wound healing; STRATUM-CORNEUM; ELECTRICAL-RESISTANCE; EFFECTIVE BARRIER; PENETRATION; INTEGRITY; INTACT; DRESSINGS; BURNS; TIO2; RAT;
D O I
10.1016/j.burns.2014.02.003
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
Silver nanoparticles (AgNPs) are increasingly applied to a wide range of materials for biomedical use. These enable a close contact with human skin, thanks to the large release of silver ions that is responsible for a broad spectrum of antimicrobial activity. Silver can permeate the skin; however, there are no data available on silver permeation through skin grafts commonly used in burns recovery. The aim of our study was to evaluate silver penetration using fresh, cryopreserved, and glycerolized human skin grafts after exposure to a suspension of AgNPs in synthetic sweat using a Franz diffusion cell apparatus for 24 h. Silver permeation profiles revealed a significantly higher permeation through glycerolized skin compared with both fresh and cryopreserved skin: 24-h silver flux penetration was 0.2 ng cm(-2)h(-1) (lag time: 8.2 h) for fresh skin, 0.3 ng cm(-2)h(-1) (lag time: 10.9 h) for cryopreserved skin, and 3.8 ng cm(-2)h(-1) (lag time: 6.3 h) for glycerolized skin. Permeation through glycerolized skin is significantly higher compared to both fresh and cryopreserved skin. This result can generate relevant clinical implications for burns treatment with products containing AgNPs. (C) 2014 Elsevier Ltd and ISBI. All rights reserved.
引用
收藏
页码:1390 / 1396
页数:7
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