Engineering multiple biological functional motifs into a blank collagen-like protein template from Streptococcus pyogenes

被引:36
作者
Peng, Yong Y. [1 ]
Stoichevska, Violet [1 ]
Schacht, Kristin [1 ]
Werkmeister, Jerome A. [1 ]
Ramshaw, John A. M. [1 ]
机构
[1] CSIRO Mat Sci & Engn, Clayton, Vic 3169, Australia
关键词
triple helix; collagen; heparin binding; integrin binding; AMINO-ACID-SEQUENCE; REGENERATIVE MEDICINE; TRIPLE-HELICES; BACTERIAL; BINDING; BIOMATERIALS; STABILITY; IDENTIFICATION; PEPTIDES; CLEAVAGE;
D O I
10.1002/jbm.a.34898
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Bacterially derived triple-helical, collagen-like proteins are attractive as potential biomedical materials. The collagen-like domain of the Scl2 protein from S. pyogenes lacks any specific binding sites for mammalian cells yet possesses the inherent structural integrity of the collagen triple-helix of animal collagens. It can, therefore, be considered as a structurally-stable blank slate into which various defined, biological sequences, derived from animal collagens, can be added by substitutions or insertions, to enable production of novel designed materials to fit specific functional requirements. In the present study, we have used site directed mutagenesis to substitute two functional sequences, one for heparin binding and the other for integrin binding, into different locations in the triple-helical structure. This provided three new constructs, two containing the single substitutions and one containing both substitutions. The stability of these constructs was marginally reduced when compared to the unmodified sequence. When compared to the unmodified bacterial collagen, both the modified collagens that contain the heparin binding site showed marked binding of fluorescently labeled heparin. Similarly, the modified collagens from both constructs containing the integrin binding site showed significant adhesion of L929 cells that are known to possess the appropriate integrin receptor. C2C12 cells that lack any appropriate integrins did not bind. These data show that bacterial collagen-like sequences can be modified to act like natural extracellular matrix collagens by inserting one or more unique biological domains with defined function. (C) 2013 Wiley Periodicals, Inc.
引用
收藏
页码:2189 / 2196
页数:8
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