The protective effect of resveratrol against dentin bonding agents-induced cytotoxicity

被引：24

作者：

Atalayin, Cigdem ^{[1
]}

Armagan, Guliz ^{[2
]}

Konyalioglu, Sibel ^{[2
]}

Kemaloglu, Hande ^{[1
]}

Tezel, Huseyin ^{[1
]}

Ergucu, Zeynep ^{[1
]}

Keser, Aysegul ^{[3
]}

Dagci, Taner ^{[3
]}

Onal, Banu ^{[1
]}

机构：

[1] Ege Univ, Dept Restorat Dent, Sch Dent, Izmir, Turkey

[2] Ege Univ, Dept Biochem, Fac Pharm, Izmir, Turkey

[3] Ege Univ, Sch Med, Dept Physiol, Izmir, Turkey

来源：

DENTAL MATERIALS JOURNAL | 2015年 / 34卷 / 06期

关键词：

Antioxidant; Cytotoxicity; Dentin bonding agent; Resveratrol; ELECTROPHORESIS COMET ASSAY; HUMAN GINGIVAL FIBROBLASTS; OXIDATIVE DNA-DAMAGE; NF-KAPPA-B; IN-VITRO; RESIN MONOMERS; CELL-CULTURES; PULP CELLS; APOPTOSIS; MUTAGENICITY;

D O I：

10.4012/dmj.2015-079

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

This study was designed to evaluate the cytotoxicity of four dentin bonding agents and the effects of an antioxidant addition. Group A: G-aerial Bond, Group B: Optibond All in One, Group C: Gluma Self Etch and Group D: Clearfil S-3 Bond were added to the medium using extract method. The cells were cultured with or without resveratrol (RES) addition. MTT, reactive oxygen species (ROS), DCF, Comet and 8-OHdG measurements were performed. The agents had a dose-dependent (1:1>1:10>1:20) cytotoxic effect. Considering 1:10 concentration; Group D at 1 h (p<0.01) and Group B and D at 24 h had the weakest cytotoxic effect (p<0.05). After RES addition, the highest cell viability was determined in Groups B+RES and D+RES at 1 h and in Groups A+RES and B+RES at 24 h (p<0.01). The dentin bonding agents induced ROS production and DNA damage regarding to their composition. However, RES addition decreased the indicated parameters.

引用

页码：766 / 773

页数：8

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