Tryptanthrin suppresses double-stranded RNA-induced CXCL10 expression via inhibiting the phosphorylation of STAT1 in human umbilical vein endothelial cells

被引：14

作者：

Kawaguchi, Shogo ^{[1
]}

Sakuraba, Hirotake ^{[2
]}

Kikuchi, Hidezumi ^{[2
]}

Numao, Noriyuki ^{[1
]}

Asari, Taka ^{[2
]}

Hiraga, Hiroto ^{[2
]}

Ding, Jiangli ^{[1
]}

Matsumiya, Tomoh ^{[1
]}

Seya, Kazuhiko ^{[1
]}

Fukuda, Shinsaku ^{[2
]}

Imaizumi, Tadaatsu ^{[1
]}

机构：

[1] Hirosaki Univ, Inst Brain Sci, Dept Vasc Biol, Grad Sch Med, 5 Zaifu Cho, Hirosaki, Aomori 0368562, Japan

[2] Hirosaki Univ, Grad Sch Med, Dept Gastroenterol & Hematol, 5 Zaifu Cho, Hirosaki, Aomori 0368562, Japan

来源：

MOLECULAR IMMUNOLOGY | 2021年 / 129卷

基金：

日本学术振兴会;

关键词：

Tryptanthrin; TLR3; STAT1; CXCL10; Vascular endothelial cells; TOLL-LIKE RECEPTOR-3; POLYGONUM-TINCTORIUM; INDIGO NATURALIS; IFN-GAMMA; ACTIVATION; INFECTION; IL-6;

D O I：

10.1016/j.molimm.2020.11.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tryptanthrin is a bioactive component of indigo plants such as Polygonum tinctrorium and known to have an anti-inflammatory activity. The aim of this study was to investigate the effects of tryptanthrin on Toll-like receptor 3 (TLR3)-mediated cytokine and chemokine expression in human umbilical vein endothelial cells (HUVEC). Herein, we found that tryptanthrin suppressed the expression of CXCL10 in HUVEC upon stimulation with a TLR3 ligand polyinosinic-polycytidylic acid (poly IC). Tryptanthrin did not inhibit poly IC-induced activation of interferon regulatory factor 3 (IRF3) or the mRNA expression of interferon (IFN)-beta, while it significantly suppressed the expression of RIG-I, MDA5, and classical IFN-stimulated genes (ISGs). Tryptanthrin attenuated the phosphorylation and nuclear translocation of STAT1 in HUVEC stimulated with not only poly IC but also recombinant IFN-beta. These results suggested that tryptanthrin inhibited poly IC-induced expression of CXCL10 and ISGs via suppressing the activation of STAT1 in HUVEC. Our findings indicate that tryptanthrin may be useful for regulating TLR3-mediated vascular inflammation.

引用

页码：32 / 38

页数：7

共 30 条

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