Separation of stereoisomers in a simulated moving bed-supercritical fluid chromatography plant

被引：47

作者：

Depta, A ^{[1
]}

Giese, T ^{[1
]}

Johannsen, M ^{[1
]}

Brunner, C ^{[1
]}

机构：

[1] Tech Univ Hamburg Harburg, Arbeitsbereich Verfahrenstech 2, D-2100 Hamburg, Germany

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 865卷 / 1-2期

关键词：

simulated moving bed chromatography; preparative chromatography; adsorption isotherms; supercritical fluid chromatography; positional isomers; phytol; toluene;

D O I：

10.1016/S0021-9673(99)00833-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The combination of two techniques, simulated moving bed (SMB) and supercritical fluid chromatography (SFC), leads to an apparatus with unique features. Besides the known advantages of the SMB process, like reduced solvent consumption and its continuity, the use of supercritical carbon dioxide as the mobile phase offers an easy product recovery by depressurizing the supercritical fluid. Details of a SMB-SFC plant are presented for the first time. Due to the large number of process parameters a simulation of the SMB process is necessary to achieve optimal operating conditions. The most important thermodynamic information for a SMB process is the adsorption isotherms. Therefore, isotherms for two phytol isomers are measured and correlated. A fast dynamic model for the simulation of SMB is used to calculate the region of complete separation taking different column configurations and the compressibility of the mobile phase into account. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：175 / 186

页数：12

共 45 条

[1]

AALTONEN O, 1998, BIOPHARM EUROPE, V8, P42

[2]

[Anonymous], J HIGH RESOLUT CHROM, DOI DOI 10.1002/JHRC.1240121003

[3] PREPARATIVE-SCALE SUPERCRITICAL-FLUID CHROMATOGRAPHY [J].

BARTLE, KD ;

BEVAN, CD ;

CLIFFORD, AA ;

JAFAR, SA ;

MALAK, N ;

VERRALL, MS .

JOURNAL OF CHROMATOGRAPHY A, 1995, 697 (1-2) :579-585

[4] PREPARATIVE SUPERCRITICAL FLUID CHROMATOGRAPHY [J].

BERGER, C ;

PERRUT, M .

JOURNAL OF CHROMATOGRAPHY, 1990, 505 (01) :37-43

[5] Separation of polar solutes by packed column supercritical fluid chromatography [J].

Berger, TA .

JOURNAL OF CHROMATOGRAPHY A, 1997, 785 (1-2) :3-33

[6]

BLEHAUT J, 1996, LC GC EUR, V9, P226

[7]

BLEHAUT J, 1996, LC GC EUR, V9, P228

[8] MODIFIER EFFECTS ON RETENTION AND PEAK SHAPE IN SUPERCRITICAL FLUID CHROMATOGRAPHY [J].

BLILIE, AL ;

GREIBROKK, T .

ANALYTICAL CHEMISTRY, 1985, 57 (12) :2239-2242

[9] USE OF A NEW PIRKLE-TYPE CHIRAL STATIONARY-PHASE IN ANALYTICAL AND PREPARATIVE SUBCRITICAL FLUID CHROMATOGRAPHY OF PHARMACEUTICAL COMPOUNDS [J].

BLUM, AM ;

LYNAM, KG ;

NICOLAS, EC .

CHIRALITY, 1994, 6 (04) :302-313

[10]

BROUGHTON DB, Patent No. 3291726

← 1 2 3 4 5 →