Scanning Electron Microscopy of Bacteria Tetrasphaera duodecadis

This study reports the characterization of the Tetrasphaera duodecadis bacteria and the techniques used therein. In order to evaluate the morphological characteristics of the T. duodecadis bacteria scanning electron microscope (SEM) was used throughout its different growth stages. These microorganisms were grown in vitamin B12 broths with 1% tryptone, 0.2% yeast extract, and 0.1% glucose. The turbidimetric method was employed for the determination of bacterial concentration and growth curve. The SEM results show small agglomerates of 0.8 +/- 0.05 mu m during the lag phase, and rod-like shapes during the exponential phase with similar shapes in the stationary phase. (c) 2014 Wiley Periodicals, Inc.