Anthelmintic Activities of Aporphine from Nelumbo nucifera Gaertn. cv. Rosa-plena against Hymenolepis nana

被引:33
作者
Lin, Rong-Jyh [1 ]
Wu, Mei-Hsuan [2 ]
Ma, Yi-Hsuan [1 ]
Chung, Li-Yu [1 ]
Chen, Chung-Yi [3 ]
Yen, Chuan-Min [1 ]
机构
[1] Kaohsiung Med Univ, Dept Parasitol, Sch Med, Coll Med, Kaohsiung 807, Taiwan
[2] Kaohsiung Med Univ, Dept Med & Appl Chem, Coll Life Sci, Kaohsiung 807, Taiwan
[3] Fooyin Univ, Dept Med Lab Sci & Biotechnol, Sch Med & Hlth Sci, Kaohsiung 83102, Taiwan
关键词
Nelumbo nucifera Gaertn; aporphine; Hymenolepis nana; Anisakis simplex; anthelmintic activity; peroxyl radical; ANISAKIS-SIMPLEX; ALKALOIDS; CONSTITUENTS; ANTIOXIDANT; FLAVONOIDS;
D O I
10.3390/ijms15033624
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Nelumbo nucifera Gaertn. cv. Rosa-plena (Nelumbonaceae), commonly known as lotus, is a perennial aquatic plant grown and consumed throughout Asia. All parts of N. nucifera have been used for various medicinal purposes in oriental medicine. From the leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena (an aquatic plant), liriodenine (1), lysicamine (2), (-)-anonaine (3), (-)-asimilobine (4), (-)-caaverine (5), (-)-N-methylasimilobine (6), (-)-nuciferine (7), (-)-nornuciferine (8), (-)-roemerine (9), 7-hydroxydehydronuciferine (10) and cepharadione B (11) were isolated and identification and anthelmintic activities of aporphine was evaluated against Anisakis simplex and Hymenolepis nana. This study found that the above constituents killed H. nana or reduced their spontaneous movements (oscillation/peristalsis). However, the above constituents at various concentrations demonstrated no larvicidal effect or ability to halt spontaneous parasite movement for 72 h against A. simplex, respectively. In addition, according to an assay of cestocidal activity against H. nana and nematocidal activity against A. simplex, we found that the above compounds showed greater lethal efficacy on H. nana than against A. simplex. Further investigation showed that these above constituents have effects against peroxyl radicals under cestocidal effect. Together, these findings suggest that these constituents of Nelumbo nucifera Gaertn. cv. Rosa-plena might be used as anthelmintic agents against H. nana.
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页码:3624 / 3639
页数:16
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