Rapid detection of anti-chromatin autoantibodies in human serum using a portable electrochemical biosensor

被引：16

作者：

Konstantinov, Konstantin N. ^{[3
]}

Sitdikov, Ravil A. ^{[2
]}

Lopez, Gabriel P. ^{[2
]}

Atanassov, Plamen ^{[2
]}

Rubin, Robert L. ^{[1
]}

机构：

[1] Univ New Mexico, Hlth Sci Ctr, Dept Mol Genet & Microbiol, Albuquerque, NM 87131 USA

[2] Univ New Mexico, Dept Chem & Nucl Engn, Albuquerque, NM 87131 USA

[3] Univ New Mexico, Hlth Sci Ctr, Dept Internal Med, Albuquerque, NM 87131 USA

来源：

BIOSENSORS & BIOELECTRONICS | 2009年 / 24卷 / 07期

基金：

美国国家科学基金会;

关键词：

Autoantibody immunoassay; Electrochemical sensor; Point-of-care biosensor; SYSTEMIC-LUPUS-ERYTHEMATOSUS; TUMOR-ASSOCIATED ANTIGENS; HORSERADISH-PEROXIDASE; AUTOIMMUNE-DISEASES; REVISED CRITERIA; ANTIBODIES; SENSORS; DNA; CLASSIFICATION; IMMUNOSENSORS;

D O I：

10.1016/j.bios.2008.09.032

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The current system employed electrochemical sensor technology for the measurement of autoantibodies in human sera. Anti-chromatin antibodies are an early and sensitive indicator of systemic lupus erythematosus (SLE) and are typically quantified using enzyme-linked immunosorbent assays (ELISA). Electrochemical detection compared favorably with ELISA using a sample of 30 SLE sera (r = 0.9), and nonspecific binding by normal serum immunoglobulin was undetectable. The electrochemical sensor assay required <20 min processing time and utilized a hand-held apparatus with a disposable electrode. These results demonstrate the applicability of this technology to the rapid measurement of a clinically relevant analyte with an apparatus of potential simplicity and low cost. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：1949 / 1954

页数：6

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