DIRECT MOLECULAR DETECTION OF PATHOGENS IN BLOOD AS SPECIFIC RULE-IN DIAGNOSTIC BIOMARKER IN PATIENTS WITH PRESUMED SEPSIS: OUR EXPERIENCE ON A HETEROGENEOUS COHORT OF PATIENTS WITH SIGNS OF INFECTIVE SYSTEMIC INFLAMMATORY RESPONSE SYNDROME
被引:17
作者:
Avolio, Manuela
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S Maria degli Angeli Reg Hosp, Dept Clin Microbiol & Virol, I-33170 Pordenone, ItalyS Maria degli Angeli Reg Hosp, Dept Clin Microbiol & Virol, I-33170 Pordenone, Italy
Avolio, Manuela
[1
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Diamante, Paola
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S Maria degli Angeli Reg Hosp, Dept Clin Microbiol & Virol, I-33170 Pordenone, ItalyS Maria degli Angeli Reg Hosp, Dept Clin Microbiol & Virol, I-33170 Pordenone, Italy
Diamante, Paola
[1
]
Modolo, Maria Luisa
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机构:
S Maria degli Angeli Reg Hosp, Dept Clin Microbiol & Virol, I-33170 Pordenone, ItalyS Maria degli Angeli Reg Hosp, Dept Clin Microbiol & Virol, I-33170 Pordenone, Italy
Modolo, Maria Luisa
[1
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De Rosa, Rita
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S Maria degli Angeli Reg Hosp, Dept Clin Microbiol & Virol, I-33170 Pordenone, ItalyS Maria degli Angeli Reg Hosp, Dept Clin Microbiol & Virol, I-33170 Pordenone, Italy
De Rosa, Rita
[1
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Stano, Paola
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S Maria degli Angeli Reg Hosp, Dept Clin Microbiol & Virol, I-33170 Pordenone, ItalyS Maria degli Angeli Reg Hosp, Dept Clin Microbiol & Virol, I-33170 Pordenone, Italy
Stano, Paola
[1
]
Camporese, Alessandro
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S Maria degli Angeli Reg Hosp, Dept Clin Microbiol & Virol, I-33170 Pordenone, ItalyS Maria degli Angeli Reg Hosp, Dept Clin Microbiol & Virol, I-33170 Pordenone, Italy
Camporese, Alessandro
[1
]
机构:
[1] S Maria degli Angeli Reg Hosp, Dept Clin Microbiol & Virol, I-33170 Pordenone, Italy
The practical value of blood cultures in the diagnosis of sepsis is impaired by a delay in the turnaround time to result and by the fact that blood culture positive can be found for only about 30% of these patients. Conventional laboratory signs of sepsis and acute phase protein biomarkers are sensitive and easy to use, but often also very nonspecific. Molecular diagnostic reflects currently the most promising avenue to decrease time to result and to influence decision making for antibiotic therapy in the septic host. In this study, we wish to highlight the impact of the LightCycler SeptiFast, a multipathogen probe-based real-time polymerase chain reaction, in the rapid etiological diagnosis of sepsis in patients with clinical and laboratory signs of bloodstream infections. We have evaluated prospectively 830 adult patients with suspected bloodstream infection and at least two criteria of systemic inflammatory response syndrome. In more than 50% of critically ill patients strongly suspected of having sepsis, we arrived to an etiological diagnosis only by the molecular method in a median time of 15 h, with specificity and predictive positive values of 96% and 94%, respectively. We highlight the role of DNAemia as time-critical, high-specificity, etiological, non-culture-based rule-in diagnostic biomarker in patients with presumed sepsis.
机构:
Inst Pasteur, Dept Infect & Epidemiol, Unit Cytokines & Inflammat, Paris, FranceInst Pasteur, Dept Infect & Epidemiol, Unit Cytokines & Inflammat, Paris, France
Fitting, Catherine
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Parlato, Marianna
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机构:
Inst Pasteur, Dept Infect & Epidemiol, Unit Cytokines & Inflammat, Paris, FranceInst Pasteur, Dept Infect & Epidemiol, Unit Cytokines & Inflammat, Paris, France
Parlato, Marianna
;
Adib-Conquy, Minou
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机构:
Inst Pasteur, Dept Infect & Epidemiol, Unit Cytokines & Inflammat, Paris, FranceInst Pasteur, Dept Infect & Epidemiol, Unit Cytokines & Inflammat, Paris, France
Adib-Conquy, Minou
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Memain, Nathalie
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h-index: 0
机构:
Delafontaine Hosp, Serv Reanimat, St Denis, FranceInst Pasteur, Dept Infect & Epidemiol, Unit Cytokines & Inflammat, Paris, France
Memain, Nathalie
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Philippart, Francois
论文数: 0引用数: 0
h-index: 0
机构:
Univ Paris 05, Grp Hosp Paris St Joseph, Serv Reanimat Med Chirurg, Paris, FranceInst Pasteur, Dept Infect & Epidemiol, Unit Cytokines & Inflammat, Paris, France
Philippart, Francois
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Misset, Benoit
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Monchi, Mehran
论文数: 0引用数: 0
h-index: 0
机构:
Jacques Cartier Hosp, Serv Soins Intensifs, Massy Palaiseau, FranceInst Pasteur, Dept Infect & Epidemiol, Unit Cytokines & Inflammat, Paris, France
Monchi, Mehran
;
Cavaillon, Jean-Marc
论文数: 0引用数: 0
h-index: 0
机构:
Inst Pasteur, Dept Infect & Epidemiol, Unit Cytokines & Inflammat, Paris, FranceInst Pasteur, Dept Infect & Epidemiol, Unit Cytokines & Inflammat, Paris, France
Cavaillon, Jean-Marc
;
Adrie, Christophe
论文数: 0引用数: 0
h-index: 0
机构:
Univ Paris 05, Cochin Hosp, Serv Physiol & Explorat Fonct, Paris, FranceInst Pasteur, Dept Infect & Epidemiol, Unit Cytokines & Inflammat, Paris, France
机构:
Ctr Hosp Lyon Sud, Bacteriol Lab, F-69495 Pierre Benite, France
Univ Lyon, F-69000 Lyon, France
Univ Lyon 1, Fac Med Lyon Sud Charles Merieux, Lab Biometrie & Biol Evolut, CNRS,UMR 5558, F-69921 Oullins, FranceCtr Hosp Lyon Sud, Bacteriol Lab, F-69495 Pierre Benite, France
Leyssene, D.
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Gardes, S.
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h-index: 0
机构:
Ctr Hosp Lyon Sud, Unite Hyg & Epidemiol, F-69495 Pierre Benite, FranceCtr Hosp Lyon Sud, Bacteriol Lab, F-69495 Pierre Benite, France
Gardes, S.
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Vilquin, P.
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h-index: 0
机构:
Ctr Hosp Lyon Sud, Unite Hyg & Epidemiol, F-69495 Pierre Benite, FranceCtr Hosp Lyon Sud, Bacteriol Lab, F-69495 Pierre Benite, France
Vilquin, P.
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Flandrois, J. -P.
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h-index: 0
机构:
Ctr Hosp Lyon Sud, Bacteriol Lab, F-69495 Pierre Benite, France
Univ Lyon, F-69000 Lyon, France
Univ Lyon 1, Fac Med Lyon Sud Charles Merieux, Lab Biometrie & Biol Evolut, CNRS,UMR 5558, F-69921 Oullins, FranceCtr Hosp Lyon Sud, Bacteriol Lab, F-69495 Pierre Benite, France
Flandrois, J. -P.
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Carret, G.
论文数: 0引用数: 0
h-index: 0
机构:
Ctr Hosp Lyon Sud, Bacteriol Lab, F-69495 Pierre Benite, France
Univ Lyon, F-69000 Lyon, France
Univ Lyon 1, Fac Med Lyon Sud Charles Merieux, Lab Biometrie & Biol Evolut, CNRS,UMR 5558, F-69921 Oullins, FranceCtr Hosp Lyon Sud, Bacteriol Lab, F-69495 Pierre Benite, France
Carret, G.
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Lamy, B.
论文数: 0引用数: 0
h-index: 0
机构:
Ctr Hosp Reg Univ, Lab Bacteriol, F-34000 Montpellier, FranceCtr Hosp Lyon Sud, Bacteriol Lab, F-69495 Pierre Benite, France
机构:
Univ Calif Davis, Dept Pathol & Lab Med, Davis, CA 95616 USA
Univ Calif Davis, Point Of Care Testing Ctr Teaching & Res, Davis, CA 95616 USAUniv Calif Davis, Dept Pathol & Lab Med, Davis, CA 95616 USA
Louie, Richard F.
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Tang, Zuping
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h-index: 0
机构:
Univ Calif Davis, Dept Pathol & Lab Med, Davis, CA 95616 USA
Univ Calif Davis, Point Of Care Testing Ctr Teaching & Res, Davis, CA 95616 USAUniv Calif Davis, Dept Pathol & Lab Med, Davis, CA 95616 USA
Tang, Zuping
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Albertson, Timothy E.
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机构:
Univ Calif Davis, Dept Med, Div Pulm & Crit Care, Davis, CA 95616 USAUniv Calif Davis, Dept Pathol & Lab Med, Davis, CA 95616 USA
Albertson, Timothy E.
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Cohen, Stuart
论文数: 0引用数: 0
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机构:
Univ Calif Davis, Dept Med, Div Infect Dis, Davis, CA 95616 USAUniv Calif Davis, Dept Pathol & Lab Med, Davis, CA 95616 USA
Cohen, Stuart
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Tran, Nam K.
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机构:
Univ Calif Davis, Dept Pathol & Lab Med, Davis, CA 95616 USA
Univ Calif Davis, Point Of Care Testing Ctr Teaching & Res, Davis, CA 95616 USAUniv Calif Davis, Dept Pathol & Lab Med, Davis, CA 95616 USA
Tran, Nam K.
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Kost, Gerald J.
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h-index: 0
机构:
Univ Calif Davis, Dept Pathol & Lab Med, Davis, CA 95616 USA
Univ Calif Davis, Point Of Care Testing Ctr Teaching & Res, Davis, CA 95616 USAUniv Calif Davis, Dept Pathol & Lab Med, Davis, CA 95616 USA
机构:
Inst Pasteur, Dept Infect & Epidemiol, Unit Cytokines & Inflammat, Paris, FranceInst Pasteur, Dept Infect & Epidemiol, Unit Cytokines & Inflammat, Paris, France
Fitting, Catherine
;
Parlato, Marianna
论文数: 0引用数: 0
h-index: 0
机构:
Inst Pasteur, Dept Infect & Epidemiol, Unit Cytokines & Inflammat, Paris, FranceInst Pasteur, Dept Infect & Epidemiol, Unit Cytokines & Inflammat, Paris, France
Parlato, Marianna
;
Adib-Conquy, Minou
论文数: 0引用数: 0
h-index: 0
机构:
Inst Pasteur, Dept Infect & Epidemiol, Unit Cytokines & Inflammat, Paris, FranceInst Pasteur, Dept Infect & Epidemiol, Unit Cytokines & Inflammat, Paris, France
Adib-Conquy, Minou
;
Memain, Nathalie
论文数: 0引用数: 0
h-index: 0
机构:
Delafontaine Hosp, Serv Reanimat, St Denis, FranceInst Pasteur, Dept Infect & Epidemiol, Unit Cytokines & Inflammat, Paris, France
Memain, Nathalie
;
Philippart, Francois
论文数: 0引用数: 0
h-index: 0
机构:
Univ Paris 05, Grp Hosp Paris St Joseph, Serv Reanimat Med Chirurg, Paris, FranceInst Pasteur, Dept Infect & Epidemiol, Unit Cytokines & Inflammat, Paris, France
Philippart, Francois
;
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机构:
Misset, Benoit
;
Monchi, Mehran
论文数: 0引用数: 0
h-index: 0
机构:
Jacques Cartier Hosp, Serv Soins Intensifs, Massy Palaiseau, FranceInst Pasteur, Dept Infect & Epidemiol, Unit Cytokines & Inflammat, Paris, France
Monchi, Mehran
;
Cavaillon, Jean-Marc
论文数: 0引用数: 0
h-index: 0
机构:
Inst Pasteur, Dept Infect & Epidemiol, Unit Cytokines & Inflammat, Paris, FranceInst Pasteur, Dept Infect & Epidemiol, Unit Cytokines & Inflammat, Paris, France
Cavaillon, Jean-Marc
;
Adrie, Christophe
论文数: 0引用数: 0
h-index: 0
机构:
Univ Paris 05, Cochin Hosp, Serv Physiol & Explorat Fonct, Paris, FranceInst Pasteur, Dept Infect & Epidemiol, Unit Cytokines & Inflammat, Paris, France
机构:
Ctr Hosp Lyon Sud, Bacteriol Lab, F-69495 Pierre Benite, France
Univ Lyon, F-69000 Lyon, France
Univ Lyon 1, Fac Med Lyon Sud Charles Merieux, Lab Biometrie & Biol Evolut, CNRS,UMR 5558, F-69921 Oullins, FranceCtr Hosp Lyon Sud, Bacteriol Lab, F-69495 Pierre Benite, France
Leyssene, D.
;
Gardes, S.
论文数: 0引用数: 0
h-index: 0
机构:
Ctr Hosp Lyon Sud, Unite Hyg & Epidemiol, F-69495 Pierre Benite, FranceCtr Hosp Lyon Sud, Bacteriol Lab, F-69495 Pierre Benite, France
Gardes, S.
;
Vilquin, P.
论文数: 0引用数: 0
h-index: 0
机构:
Ctr Hosp Lyon Sud, Unite Hyg & Epidemiol, F-69495 Pierre Benite, FranceCtr Hosp Lyon Sud, Bacteriol Lab, F-69495 Pierre Benite, France
Vilquin, P.
;
Flandrois, J. -P.
论文数: 0引用数: 0
h-index: 0
机构:
Ctr Hosp Lyon Sud, Bacteriol Lab, F-69495 Pierre Benite, France
Univ Lyon, F-69000 Lyon, France
Univ Lyon 1, Fac Med Lyon Sud Charles Merieux, Lab Biometrie & Biol Evolut, CNRS,UMR 5558, F-69921 Oullins, FranceCtr Hosp Lyon Sud, Bacteriol Lab, F-69495 Pierre Benite, France
Flandrois, J. -P.
;
Carret, G.
论文数: 0引用数: 0
h-index: 0
机构:
Ctr Hosp Lyon Sud, Bacteriol Lab, F-69495 Pierre Benite, France
Univ Lyon, F-69000 Lyon, France
Univ Lyon 1, Fac Med Lyon Sud Charles Merieux, Lab Biometrie & Biol Evolut, CNRS,UMR 5558, F-69921 Oullins, FranceCtr Hosp Lyon Sud, Bacteriol Lab, F-69495 Pierre Benite, France
Carret, G.
;
Lamy, B.
论文数: 0引用数: 0
h-index: 0
机构:
Ctr Hosp Reg Univ, Lab Bacteriol, F-34000 Montpellier, FranceCtr Hosp Lyon Sud, Bacteriol Lab, F-69495 Pierre Benite, France
机构:
Univ Calif Davis, Dept Pathol & Lab Med, Davis, CA 95616 USA
Univ Calif Davis, Point Of Care Testing Ctr Teaching & Res, Davis, CA 95616 USAUniv Calif Davis, Dept Pathol & Lab Med, Davis, CA 95616 USA
Louie, Richard F.
;
Tang, Zuping
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Pathol & Lab Med, Davis, CA 95616 USA
Univ Calif Davis, Point Of Care Testing Ctr Teaching & Res, Davis, CA 95616 USAUniv Calif Davis, Dept Pathol & Lab Med, Davis, CA 95616 USA
Tang, Zuping
;
Albertson, Timothy E.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Med, Div Pulm & Crit Care, Davis, CA 95616 USAUniv Calif Davis, Dept Pathol & Lab Med, Davis, CA 95616 USA
Albertson, Timothy E.
;
Cohen, Stuart
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Med, Div Infect Dis, Davis, CA 95616 USAUniv Calif Davis, Dept Pathol & Lab Med, Davis, CA 95616 USA
Cohen, Stuart
;
Tran, Nam K.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Pathol & Lab Med, Davis, CA 95616 USA
Univ Calif Davis, Point Of Care Testing Ctr Teaching & Res, Davis, CA 95616 USAUniv Calif Davis, Dept Pathol & Lab Med, Davis, CA 95616 USA
Tran, Nam K.
;
Kost, Gerald J.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Pathol & Lab Med, Davis, CA 95616 USA
Univ Calif Davis, Point Of Care Testing Ctr Teaching & Res, Davis, CA 95616 USAUniv Calif Davis, Dept Pathol & Lab Med, Davis, CA 95616 USA