Blood Collection Through Subclavian Vein Puncture in Mice

被引：6

作者：

Yang, Hui ^{[1
]}

Wu, Chenlu ^{[1
]}

Liu, Fengjiao ^{[2
]}

Wang, Mi ^{[1
]}

Zou, Pu ^{[1
]}

He, Yuhu ^{[1
]}

Liu, Qiming ^{[1
]}

Zhou, Qin ^{[3
]}

Zhou, Shenghua ^{[1
]}

机构：

[1] Cent S Univ, Xiangya Hosp 2, Dept Cardiol, Changsha, Hunan, Peoples R China

[2] Cent S Univ, Xiangya Hosp, Dept Cardiol, Changsha, Hunan, Peoples R China

[3] Cent S Univ, Xiangya Hosp 2, Dept Gen Surg, Changsha, Hunan, Peoples R China

来源：

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2019年 / 147期

基金：

中国国家自然科学基金;

关键词：

Medicine; Issue; 147; blood collection; subclavian vein; puncture; Mouse; superior sternal fossa; orbital artery; WELFARE; IMPACT;

D O I：

10.3791/59556

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The mouse is the foremost mammalian model for studying human disease and human health. However, blood sample collection from mice is challenging in research work. Tail blood collection is a popular method when a small amount of blood sample is needed. Orbital artery could be considered if a large amount of blood is needed but this blood collection method has ethical issues. Formerly, we demonstrated the feasibility and safety of blood sample collection through subclavian vein puncture in rats, and here we investigate whether this method could be used in mice. We report that this method is safe and practical for blood collection in mice. Blood collection through the subclavian vein puncture in mice can be a convenient method in daily research works.

引用

页数：6

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