Oligostilbenoids in Vitis vinifera L. Pinot Noir grape cane extract: Isolation, characterization, in vitro antioxidant capacity and anti-proliferative effect on cancer cells

被引：62

作者：

Saez, Vania ^{[1
]}

Pastene, Edgar ^{[2
]}

Vergara, Carola ^{[1
]}

Mardones, Claudia ^{[1
]}

Hermosin-Gutierrez, Isidro ^{[3
]}

Gomez-Alonso, Sergio ^{[3
]}

Victoria Gomez, M. ^{[3
]}

Theoduloz, Cristina ^{[4
]}

Riquelme, Sebastian ^{[5
]}

von Baer, Dietrich ^{[1
]}

机构：

[1] Univ Concepcion, Fac Farm, Dept Anal Instrumental, POB 160-C, Concepcion, Chile

[2] Univ Concepcion, Fac Farm, Lab Farmacognosia, POB 160-C, Concepcion 4070386, Chile

[3] Univ Castilla La Mancha, Inst Reg Invest Cient Aplicada, Campus Univ S-N, E-13071 Ciudad Real, Spain

[4] Univ Talca, Fac Ciencias Salud, Lab Cult Celular, POB 747, Talca 3460000, Chile

[5] Univ Concepcion, UDT, Coronel, Chile

来源：

FOOD CHEMISTRY | 2018年 / 265卷

关键词：

Grape cane; Oligostilbenoids; (E)-Resveratrol; Antioxidant capacity; Antiproliferative effect; RESVERATROL OLIGOMERS; LUNG-CANCER; STILBENOIDS; PURIFICATION; STORAGE; WASTE; STEMS; ORAC;

D O I：

10.1016/j.foodchem.2018.05.050

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The following oligostilbenoids were isolated from extracts of Vitis vinifera L. Pinot Noir grape canes produced at a pilot-plant scale: (E)-epsilon-viniferin, (E)-resveratrol, (E)-piceatannol, ampelopsin A, vitisin B, pallidol, (E)-delta-viniferin, (E)-omega-viniferin, (E)-trans-cis-miyabenol C, isorhapontigenin, scirpusin A, and a new isomer named iso-scirpusin A. The antioxidant capacity of the isolated stilbenoids was studied by three different assays, and their 50% inhibition concentration (IC50) against cancer cells was determined by MTT reduction assay. Besides (E)resveratrol, stilbenoids have outstanding antioxidant capacity in the ORAC-FL assay. The strongest anti-proliferative effect was observed for (E)-piceatannol and ampelopsin A against the bladder cancer cell line J82. (E)-Piceatannol has inhibitory effect on human lung cancer SK-MES-1 cells. Moreover, the whole extract has antiproliferative effect on all tested cell lines. In conclusion, beside (E)-resveratrol, grape cane extract contains oligostilbenoids with potential health benefits. This underexploited viticultural residue has the potential to produce valuable phytochemicals or ingredients in functional foods.

引用

页码：101 / 110

页数：10